PRP24 U6 snRNP complex subunit PRP24 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PRP24
Official Full Name: U6 snRNP complex subunit PRP24
Primary source: SGD:S000004881
Locus tag: YMR268C
See related: AllianceGenome:SGD:S000004881; FungiDB:YMR268C; VEuPathDB:YMR268C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables U6 snRNA binding activity. Involved in spliceosomal complex assembly and spliceosomal tri-snRNP complex assembly. Part of U6 snRNP. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See PRP24 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (802888..804222, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that U6 snRNP complex subunit Prp24 binds U6 snRNA with much higher affinity than U4 snRNA.
Title: Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.
results suggest that the autophagy and proteasomal pathways degrade distinct translation and RNA turnover factors in a Ubp3p-dependent manner during nitrogen starvation.
Title: Spliceosome assembly in the absence of stable U4/U6 RNA pairing.
The crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein is presented.
Title: Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution.
Nearly complete backbone and side chain resonance assignments have been obtained for the second RNA recognition motif (RRM) of Prp24 (RRM2, residues M114-E197) both in isolation and bound to a six nucleotide fragment of U6 RNA (AGAGAU).
Title: (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA.
RNA recognition motifs (RRMs) 1 and 2 of yeast Prp24 bind the same central site in U6 RNA that is bound by the two RRMs of human Prp24, and that RRMs 3 and 4 bind lower affinity flanking sites, thereby restricting the stoichiometry of Prp24 binding
Title: The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding.
structure of the RNA recognition motifs of splicing factor prp24.
Title: Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables U6 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables snRNA binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spliceosomal tri-snRNP complex assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of U6 snRNP	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: U6 snRNP complex subunit PRP24

NP_013995.1

Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

802888..804222 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182775.1 → NP_013995.1 TPA: U6 snRNP complex subunit PRP24 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013995.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W094, E9P966, P49960

UniProtKB/TrEMBL

B3LMF5, B5VQ32, C7GR86, C8ZFD4, G2WKX6

Conserved Domains (5) summary

pfam05391
Location:425 → 443

Lsm_interact; Lsm interaction motif

cd12296
Location:41 → 113

RRM1_Prp24; RNA recognition motif 1 in fungal pre-messenger RNA splicing protein 24 (Prp24) and similar proteins

cd12297
Location:117 → 195

RRM2_Prp24; RNA recognition motif 2 in fungal pre-messenger RNA splicing protein 24 (Prp24) and similar proteins

cd12298
Location:210 → 286

RRM3_Prp24; RNA recognition motif 3 in fungal pre-messenger RNA splicing protein 24 (Prp24) and similar proteins

cd12299
Location:312 → 386

RRM4_Prp24; RNA recognition motif 4 in fungal pre-messenger RNA splicing protein 24 (Prp24) and similar proteins