RAD50 MRX complex DNA-binding subunit [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RAD50
Gene description: MRX complex DNA-binding subunit
Primary source: FungiDB:YNL250W
Locus tag: YNL250W
See related: SGD:S000005194; AllianceGenome:SGD:S000005194; VEuPathDB:YNL250W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ATP hydrolysis activity; DNA binding activity; and adenylate kinase activity. Involved in DNA metabolic process; homologous chromosome pairing at meiosis; and negative regulation of endodeoxyribonuclease activity. Located in nucleus. Part of Mre11 complex. Human ortholog(s) of this gene implicated in transitional cell carcinoma. Orthologous to human RAD50 (RAD50 double strand break repair protein). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RAD50 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (175410..179348)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.
Title: Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.
Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex.
Title: Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex.
Inhibition of MRN activity by a telomere protein motif.
Title: Inhibition of MRN activity by a telomere protein motif.
A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
Title: A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
Effect of BRCA1 missense variants on gene reversion in DNA double-strand break repair mutants and cell cycle-arrested cells of Saccharomyces cerevisiae.
Title: Effect of BRCA1 missense variants on gene reversion in DNA double-strand break repair mutants and cell cycle-arrested cells of Saccharomyces cerevisiae.
Here we describe two separation-of-functions alleles, mre11-S499P and rad50-A78T, which we show to specifically affect Tel1 activation without impairing MRX functions in DSB repair. Both Mre11-S499P and Rad50-A78T reduce Tel1-MRX interaction leading to poor Tel1 association at DSBs and consequent loss of Tel1 activation
Title: The ATP-bound conformation of the Mre11-Rad50 complex is essential for Tel1/ATM activation.
Data show that ssDNA endodeoxyribonuclease SAE2 (Sae2) cooperates with the Mre11-Rad50-Xrs2 (MRX) complex to initiate resection of DNA double-strand breaks (DSBs) and to maintain the DSB ends in close proximity to allow their repair.
Title: Structurally distinct Mre11 domains mediate MRX functions in resection, end-tethering and DNA damage resistance.
Phosphorylation of ssDNA endodeoxyribonuclease SAE2 (Sae2)does not affect its overall capacity to interact with Mre11-Rad50-Xrs2 (MRX), it controls the specific physical and functional interaction with the MRX subunit Rad50, which is a prerequisite for the regulation of the Mre11 nuclease.
Title: Regulatory control of DNA end resection by Sae2 phosphorylation.
Xrs2 dependent and independent functions of the Mre11-Rad50 complex orchestrating the cellular response to double-strand DNA breaks have been described.
Title: Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex.
The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination.
Title: The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination.

Submit: New GeneRIF Correction See all GeneRIFs (33)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables adenylate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables adenylate kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome organization involved in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in homologous chromosome pairing at meiosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of DNA trinucleotide repeats	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleoside monophosphate phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance via recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via telomerase	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Mre11 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Mre11 complex	IEA Inferred from Electronic Annotation more info
part_of Mre11 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in condensed nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: MRX complex DNA-binding subunit

NP_014149.1

Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress