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SLA2 Sla2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855478, updated on 28-Oct-2024

Summary

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Official Symbol
SLA2
Official Full Name
Sla2p
Primary source
SGD:S000005187
Locus tag
YNL243W
See related
AllianceGenome:SGD:S000005187; FungiDB:YNL243W; VEuPathDB:YNL243W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
END4; MOP2
Summary
Predicted to enable several functions, including anion binding activity; clathrin adaptor activity; and clathrin light chain binding activity. Involved in actin cytoskeleton organization; endocytosis; and negative regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch and incipient cellular bud site. Orthologous to human HIP1 (huntingtin interacting protein 1) and HIP1R (huntingtin interacting protein 1 related). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See SLA2 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
1
Sequence:
Chromosome: XIV; NC_001146.8 (188051..190957)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Vps75p Neighboring gene U2-type spliceosomal complex subunit CWC25 Neighboring gene translation initiation factor eIF1 Neighboring gene Atg2p Neighboring gene glucose-6-phosphate dehydrogenase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Interaction of Sla2p's ANTH domain with PtdIns(4,5)P2 is important for actin-dependent endocytic internalization. Sun Y, et al. Mol Biol Cell, 2005 Feb. PMID 15574875, Free PMC Article
  2. Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals. Yang S, et al. Mol Biol Cell, 1999 Jul. PMID 10397764, Free PMC Article
  3. Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein. Marx U, et al. Eur J Biochem, 1999 Jul. PMID 10429211
  4. The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals. McCann RO, et al. Proc Natl Acad Sci U S A, 1997 May 27. PMID 9159132, Free PMC Article
  5. end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. Raths S, et al. J Cell Biol, 1993 Jan. PMID 8380177, Free PMC Article

See all (155) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
    Title: Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
  2. Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton and (2) hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission to endocytosis sites
    Title: TORC2 controls endocytosis through plasma membrane tension.
  3. analysis of the molecular mechanism of plasma membrane-actin cytoskeleton coupling mediated by cooperative action of epsin Ent1 and the HIP1R homolog Sla2 in yeast Saccharomyces cerevisiae
    Title: Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis.
  4. CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization
    Title: Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin.
  5. growth defects in cells lacking SLA2 are associated with a cell cycle delay that is influenced by Swe1, a morphogenesis checkpoint kinase
    Title: SLA2 mutations cause SWE1-mediated cell cycle phenotypes in Candida albicans and Saccharomyces cerevisiae.
  6. Two recessive mutations, act1-301 in the actin gene and sla2-82 in a gene involved in cortical actin patch assembly, were identified.
    Title: Identification of novel mutations in ACT1 and SLA2 that suppress the actin-cable-overproducing phenotype caused by overexpression of a dominant active form of Bni1p in Saccharomyces cerevisiae.
  7. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation.
    Title: Novel function of clathrin light chain in promoting endocytic vesicle formation.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables actin binding IEA
Inferred from Electronic Annotation
more info
  
enables actin filament binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables clathrin adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables clathrin binding IEA
Inferred from Electronic Annotation
more info
  
enables clathrin light chain binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables molecular_function ND
No biological Data available
more info
  
enables phosphatidylinositol-3,4-bisphosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphatidylinositol-3,5-bisphosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phospholipid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in actin cortical patch assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin filament organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in clathrin coat assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in actin cortical patch IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in actin cortical patch IDA
Inferred from Direct Assay
more info
 PubMed 
located_in actin cortical patch IEA
Inferred from Electronic Annotation
more info
  
located_in cell cortex IEA
Inferred from Electronic Annotation
more info
  
located_in cellular bud neck HDA PubMed 
located_in cellular bud tip IEA
Inferred from Electronic Annotation
more info
  
is_active_in clathrin-coated vesicle IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cortical actin cytoskeleton IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in incipient cellular bud site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mating projection tip HDA PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Sla2p
NP_014156.2
  • Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    188051..190957
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183081.2 → NP_014156.2  TPA: Sla2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014156.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W0V0, P33338, Q02434
    UniProtKB/TrEMBL
    B3LP82, C7GPB8, C8ZFZ3, N1P530
    Conserved Domains (3) summary
    smart00307
    Location:763 → 965
    ILWEQ; I/LWEQ domain
    COG1196
    Location:420 → 546
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    pfam07651
    Location:7 → 269
    ANTH; ANTH domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P33338.5

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