SRP1 karyopherin alpha [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SRP1
Official Full Name: karyopherin alpha
Primary source: SGD:S000005133
Locus tag: YNL189W
See related: AllianceGenome:SGD:S000005133; FungiDB:YNL189W; VEuPathDB:YNL189W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KAP60; SCM1
Summary: Enables nuclear import signal receptor activity. Involved in NLS-bearing protein import into nucleus; proteasome localization; and protein targeting to membrane. Located in cytoplasm and nucleus. Part of NLS-dependent protein nuclear import complex. Orthologous to several human genes including KPNA1 (karyopherin subunit alpha 1) and KPNA6 (karyopherin subunit alpha 6). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SRP1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (284260..285888)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

binding of Yar1 to one N-domain and binding of Kap60 to the second N-domain of dimerized Rps3 orchestrates import and protection of the ribosomal protein
Title: Nuclear import of dimerized ribosomal protein Rps3 in complex with its chaperone Yar1.
Data suggest that, in nuclear exosomes, karyopherins are bound to the Rrp6 complex; Srp1, Kap95, and Sxm1 appear to be the most important karyopherins for nuclear import of Rrp6. (Rrp6 = exosome nuclease subunit RRP6; Srp1 = karyopherin alpha kap60; Kap95 = karyopherin beta Kap95; Sxm1 = karyopherin Kap108)
Title: Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae.
Srp1 has a role in performing distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus
Title: Yeast importin-α (Srp1) performs distinct roles in the import of nuclear proteins and in targeting proteasomes to the nucleus.
This study unveils a previously unknown role for Srp1 and Sts1 in cotranslational protein degradation and suggests a novel model whereby Srp1 and Sts1 cooperate to couple proteasomes to ribosome-bound nascent polypeptides.
Title: Nuclear import factor Srp1 and its associated protein Sts1 couple ribosome-bound nascent polypeptides to proteasomes for cotranslational degradation.
Hxk2 as a new cargo for the alpha/beta-importin pathway of S. cerevisiae.
Title: Nuclear import of the yeast hexokinase 2 protein requires α/β-importin-dependent pathway.
This report provides the first evidence that isoforms of Kap60 exist in yeast, and these isoforms participate in the molecular sorting and enrichment of inner nuclear membrane proteins to the nuclear envelope.
Title: Truncated isoforms of Kap60 facilitate trafficking of Heh2 to the nuclear envelope.
Pct1 interacts with Kap60 and Kap95 with this tripartite complex transiting the nuclear pore.
Title: The Kap60-Kap95 karyopherin complex directly regulates phosphatidylcholine synthesis.
These results reveal that the classical nuclear protein import pathway makes important contributions to the G(1)/S cell cycle transition.
Title: The classical nuclear localization signal receptor, importin-alpha, is required for efficient transition through the G1/S stage of the cell cycle in Saccharomyces cerevisiae.
There is a correlation between the binding affinity of an NLS cargo for the NLS receptor, importin alpha, and the import rate for this cargo.
Title: Nuclear localization signal receptor affinity correlates with in vivo localization in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables nuclear import signal receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear import signal receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables nuclear import signal receptor activity	IEA Inferred from Electronic Annotation more info
enables nuclear localization sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in NLS-bearing protein import into nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in NLS-bearing protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	IEA Inferred from Electronic Annotation more info
involved_in protein targeting to membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of NLS-dependent protein nuclear import complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: karyopherin alpha

NP_014210.1

Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

284260..285888

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183027.1 → NP_014210.1 TPA: karyopherin alpha [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014210.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0Z8, Q02821

UniProtKB/TrEMBL

A6ZRP8, B3LP38, B5VQM0, C7GTG1, C8ZG41, G2WLS1, N1NXR4

Conserved Domains (2) summary

COG5064
Location:8 → 542

SRP1; Karyopherin (importin) alpha [Intracellular trafficking and secretion]

sd00043
Location:294 → 326

ARM; armadillo repeat [structural motif]