DMA2 ubiquitin-conjugating protein DMA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DMA2
Official Full Name: ubiquitin-conjugating protein DMA2
Primary source: SGD:S000005060
Locus tag: YNL116W
See related: AllianceGenome:SGD:S000005060; FungiDB:YNL116W; VEuPathDB:YNL116W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CHF2
Summary: Enables ubiquitin-protein transferase activity. Involved in several processes, including cellular protein metabolic process; cytoskeleton organization; and mitotic spindle orientation checkpoint signaling. Located in cellular bud neck split septin rings and cellular bud tip. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See DMA2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (408341..409909)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Catalysis-dependent and redundant roles of Dma1 and Dma2 in maintenance of genome stability in Saccharomyces cerevisiae.
Title: Catalysis-dependent and redundant roles of Dma1 and Dma2 in maintenance of genome stability in Saccharomyces cerevisiae.
Dma1/2 mediate rapid Sld2 degradation by recognizing a complex phospho-degron
Title: Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2.
Expression of a hyperactive variant of Bni1 (Bni1-V360D) rescues these defects and partially restores proper spindle positioning in the mutant, suggesting that the failure of dma1 dma2 mutant cells to position the spindle is partly due to faulty formin activity.
Title: Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.
These results suggest that Pog1 controls the cell cycle and its phosphorylated form is downregulated by Dma2.
Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae.
Dma1 and Dma2 control septin dynamics and the SPOC by promoting the efficient recruitment of Elm1 to the bud neck
Title: Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck.
the Cdc123-Chf-Gcd11 axis is an essential pathway for nutritional control of START that runs parallel to the Tor-Gcn2-Sui2 system of translational control
Title: Cdc123 and checkpoint forkhead associated with RING proteins control the cell cycle by controlling eIF2gamma abundance.
Dma2 is required to ensure timely mitotic exit network activation in telophase.
Title: Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cellular bud neck septin ring organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of mitotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle orientation checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein autoubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to bud neck	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of formin-nucleated actin cable assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of formin-nucleated actin cable assembly	IPI Inferred from Physical Interaction more info	PubMed
involved_in septin ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell division site	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck split septin rings	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cellular bud tip	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ubiquitin-conjugating protein DMA2

NP_014283.3

EC 2.3.2.27

Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

408341..409909

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182954.3 → NP_014283.3 TPA: ubiquitin-conjugating protein DMA2 [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZRW7, B0KZS8, B0KZU6, B0KZW4, B0L009, D6W166, P53924, Q66GT0

UniProtKB/TrEMBL

N1NZD5

Conserved Domains (2) summary

COG1716
Location:228 → 417

FHA; Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms]

cd16458
Location:431 → 477

RING-H2_Dmap_like; RING finger, H2 subclass, found in defective in mitotic arrest proteins (Dmap) and similar proteins