DOM34 ribosome dissociation factor DOM34 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DOM34
Official Full Name: ribosome dissociation factor DOM34
Primary source: SGD:S000004946
Locus tag: YNL001W
See related: AllianceGenome:SGD:S000004946; FungiDB:YNL001W; VEuPathDB:YNL001W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA endonuclease activity. Contributes to ribosome binding activity. Involved in RNA catabolic process; positive regulation of translation; and ribosome disassembly. Located in cytoplasm. Part of Dom34-Hbs1 complex. Orthologous to human PELO (pelota mRNA surveillance and ribosome rescue factor). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See DOM34 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (627456..628616)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34.Hbs1.GTP complex during Dom34-mediated mRNA surveillance pathways
Title: Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Translational effects of Dom34 required the 5'-UTR of the PMT1 transcript, which bound recombinant Dom34 directly at a CA/AC-rich sequence and regulated in vitro translation. Polysomal profiling revealed that Dom34 stimulates general translation moderately, but that it is especially required for translation of transcripts encoding Pmt isoforms 1, 4 and 6.
Title: Dom34 Links Translation to Protein O-mannosylation.
Study found that Dom34 rescues ribosomes that stall at the 3' end of mRNAs that are truncated. Dom34 rescues ribosomes in the 3' untranslated region of many genes. Surprisingly, many ribosomes populate this noncoding region, and these ribosomes seem to move along the mRNA without recognizing codons, presumably not translating, and eventually arrest.
Title: Dom34 rescues ribosomes in 3' untranslated regions.
Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.
Title: Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.
Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates.
Title: Dom34:hbs1 plays a general role in quality-control systems by dissociation of a stalled ribosome at the 3' end of aberrant mRNA.
The close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled the authors to propose a model for the ribosome-bound eRF1-eRF3 complex.
Title: Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.
The slow growth of cells lacking Dom34 and haploinsufficient for a protein of the 40S subunit is caused by a severe shortage of 40S subunits available for translation initiation.
Title: Why Dom34 stimulates growth of cells with defects of 40S ribosomal subunit biosynthesis.
findings show that Dom34:Hbs1 (two interacting factors genetically implicated in no-go decay) interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off
Title: Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
Results identify important functional regions of Dom34 and suggest that the proposed endonuclease activity of Dom34 is not required for mRNA cleavage in NGD.
Title: Analysis of Dom34 and its function in no-go decay.
Dom34 has a role in an mRNA decay pathway called No-Go decay and the interaction of Dom34 with Hbs1 results in an increase in the affinity constant of Hbs1 for GTP but not GDP
Title: Structure of yeast Dom34: a protein related to translation termination factor Erf1 and involved in No-Go decay.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
contributes_to ribosome binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in RNA surveillance	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in gene expression	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in nonfunctional rRNA decay	IBA Inferred from Biological aspect of Ancestor more info
involved_in nonfunctional rRNA decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IEA Inferred from Electronic Annotation more info
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, non-stop decay	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in ribosome disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in ribosome disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in ribosome disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Dom34-Hbs1 complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ribosome dissociation factor DOM34

NP_014397.1

Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication