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SPT14 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855928, updated on 22-Aug-2024

Summary

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Official Symbol
SPT14
Primary source
FungiDB:YPL175W
Locus tag
YPL175W
See related
SGD:S000006096; AllianceGenome:SGD:S000006096; VEuPathDB:YPL175W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
CWH6; GPI3
Summary
Enables phosphatidylinositol N-acetylglucosaminyltransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Located in endoplasmic reticulum. Human ortholog(s) of this gene implicated in multiple congenital anomalies-hypotonia-seizures syndrome 2 and paroxysmal nocturnal hemoglobinuria. Orthologous to human PIGA (phosphatidylinositol glycan anchor biosynthesis class A). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SPT14 in Genome Data Viewer
Location:
chromosome: XVI
Exon count:
2
Sequence:
Chromosome: XVI; NC_001148.4 (218630..220088)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene Cup9p Neighboring gene Tre1p Neighboring gene Nip100p Neighboring gene mitochondrial 54S ribosomal protein YmL40

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly. Kostova Z, et al. Eur J Biochem, 2003 Nov. PMID 14622279
  2. Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis. Kostova Z, et al. Biochem J, 2000 Sep 15. PMID 10970797, Free PMC Article
  3. Restrictive glycosylphosphatidylinositol anchor synthesis in cwh6/gpi3 yeast cells causes aberrant biogenesis of cell wall proteins. Vossen JH, et al. J Bacteriol, 1997 Apr. PMID 9079905, Free PMC Article
  4. The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis. Schönbächler M, et al. EMBO J, 1995 Apr 18. PMID 7737116, Free PMC Article
  5. Identification of SPT14/CWH6 as the yeast homologue of hPIG-A, a gene involved in the biosynthesis of GPI anchors. Vossen JH, et al. Biochim Biophys Acta, 1995 Apr 13. PMID 7727533

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylinositol N-acetylglucosaminyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphatidylinositol N-acetylglucosaminyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylinositol N-acetylglucosaminyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in GPI anchor biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in GPI anchor biosynthetic process IC
Inferred by Curator
more info
 PubMed 
involved_in GPI anchor biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
part_of glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
phosphatidylinositol N-acetylglucosaminyltransferase SPT14
NP_015150.2
  • UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    218630..220088
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183989.1 → NP_015150.2  TPA: phosphatidylinositol N-acetylglucosaminyltransferase SPT14 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015150.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    B3LKQ3, B5VSZ6, D6W3J3, P32363, Q08918
    UniProtKB/TrEMBL
    C8ZIK3, G2WP07, N1P2P7
    Conserved Domains (1) summary
    cd03796
    Location:4 → 408
    GT4_PIG-A-like; phosphatidylinositol N-acetylglucosaminyltransferase subunit A and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32363.4

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