NIP100 Nip100p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: NIP100
Gene description: Nip100p
Primary source: FungiDB:YPL174C
Locus tag: YPL174C
See related: SGD:S000006095; AllianceGenome:SGD:S000006095; VEuPathDB:YPL174C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAC13
Summary: Predicted to enable microtubule plus-end binding activity. Involved in establishment of mitotic spindle orientation. Part of dynactin complex. Colocalizes with spindle pole body. Orthologous to several human genes including CLIP1 (CAP-Gly domain containing linker protein 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See NIP100 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (220167..222773, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150(Glued) subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22
Title: Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1.
interactions of a yeast ortholog of N-IC (N-Pac11) with yeast LC8 (Dyn2) or with the intermediate chain-binding subunit of yeast dynactin (Nip100).
Title: Interactions of Yeast Dynein with Dynein Light Chain and Dynactin: GENERAL IMPLICATIONS FOR INTRINSICALLY DISORDERED DUPLEX SCAFFOLDS IN MULTIPROTEIN ASSEMBLIES.
Data suggest that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex.
Title: Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment.
Enhancement of dynein processivity by dynactin does not require the microtubule binding domains of Nip100
Title: Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin.
Dynein/dynactin helps to generate a spindle pole separating force in Saccharomyces cerevisiae.
Title: The Saccharomyces cerevisiae homolog of p24 is essential for maintaining the association of p150Glued with the dynactin complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration involved in conjugation with cellular fusion	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cell tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of dynactin complex	IDA Inferred from Direct Assay more info	PubMed
part_of dynein complex	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
part_of microtubule associated complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in spindle	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Nip100p

NP_015151.1

Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

220167..222773 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183988.1 → NP_015151.1 TPA: Nip100p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015151.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3J4, P33420, Q08917

UniProtKB/TrEMBL

A0A8H4BVQ0, C7GJC4, N1NWP6

Conserved Domains (1) summary

COG5244
Location:12 → 828

NIP100; Dynactin complex subunit involved in mitotic spindle partitioning in anaphase B [Cell cycle control, cell division, chromosome partitioning]