SMK1 mitogen-activated protein kinase SMK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SMK1
Official Full Name: mitogen-activated protein kinase SMK1
Primary source: SGD:S000006258
Locus tag: YPR054W
See related: AllianceGenome:SGD:S000006258; FungiDB:YPR054W; VEuPathDB:YPR054W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables MAP kinase activity. Acts upstream of or within ascospore wall assembly and protein localization to ascospore wall. Located in prospore membrane leading edge. Orthologous to human MAPK7 (mitogen-activated protein kinase 7) and NLK (nemo like kinase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SMK1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (666280..667446)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A fragment of Ssp2 that is sufficient to activate Smk1 contains two segments that resemble RNA recognition motifs (RRMs). Mutations in either of these motifs eliminated Ssp2's ability to activate Smk1. In contrast, deletions and insertions within the segment linking the RRM-like motifs only partially reduced the activity of Ssp2.
Title: RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.
The T residue in Smk1's activation motif is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1.
Title: Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation.
Ime2 is a key activator of Smk1 signaling.
Title: The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.
negative regulation of GSC2 by SMK1 is important for spore wall deposition
Title: The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables MAP kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ascospore wall assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within ascospore wall assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein localization to ascospore wall	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in sporulation resulting in formation of a cellular spore	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in prospore membrane leading edge	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mitogen-activated protein kinase SMK1

NP_015379.1

EC 2.7.11.24

Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for prospore membrane development and the production of outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p; activated by Cak1p-mediated phosphorylation of threonine (T207) in the activation loop during meiosis I followed by Ssp2p-promoted autophosphorylation of tyrosine (T209) during meiosis II

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

666280..667446

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184151.1 → NP_015379.1 TPA: mitogen-activated protein kinase SMK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015379.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W460, P41808

UniProtKB/TrEMBL

B3LLA9, C7GY41, C8ZJ79, G2WPM8, N1NXK0

Conserved Domains (1) summary

cl21453
Location:37 → 376

PKc_like; Protein Kinases, catalytic domain