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WSS1 metalloendopeptidase WSS1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856536, updated on 2-Nov-2024

Summary

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Official Symbol
WSS1
Official Full Name
metalloendopeptidase WSS1
Primary source
SGD:S000001176
Locus tag
YHR134W
See related
AllianceGenome:SGD:S000001176; FungiDB:YHR134W; VEuPathDB:YHR134W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables SUMO binding activity; SUMO ligase activity; and metalloendopeptidase activity. Involved in DNA repair; histone catabolic process; and protein sumoylation. Located in fungal-type vacuole and nuclear envelope. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See WSS1 in Genome Data Viewer
Location:
chromosome: VIII
Exon count:
1
Sequence:
Chromosome: VIII; NC_001140.6 (371749..372558)

Chromosome VIII - NC_001140.6Genomic Context describing neighboring genes Neighboring gene phosphatase regulator Neighboring gene Nsg1p Neighboring gene serine/threonine protein kinase YCK1 Neighboring gene Spl2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural analysis of Wss1 protein from saccharomyces cerevisiae. Yang X, et al. Sci Rep, 2017 Aug 15. PMID 28811590, Free PMC Article
  2. The Saccharomyces cerevisiae Wss1 protein is only present in mother cells. van Heusden GP, et al. FEMS Microbiol Lett, 2008 May. PMID 18336552
  3. The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response. Svoboda M, et al. DNA Repair (Amst), 2019 Aug. PMID 31276951
  4. DNA-protein crosslink repair: proteases as DNA repair enzymes. Stingele J, et al. Trends Biochem Sci, 2015 Feb. PMID 25496645
  5. Cleavage-defective Topoisomerase I mutants sharply increase G-quadruplex-associated genomic instability. Berroyer A, et al. Microb Cell, 2022 Mar 7. PMID 35291312, Free PMC Article

See all (68) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response.
    Title: The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response.
  2. e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs
    Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks.
  3. Upon DNA damage Wss1/Cdc48/Doa1 ternary complex is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity.
    Title: Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates.
  4. Data suggest that Wss1 contributes to survival of DNA-protein crosslink (DPC)-harboring cells by acting on DPCs proteolytically. Wss1 enables replication of DPC-containing DNA and thus promotes genome integrity.
    Title: A DNA-dependent protease involved in DNA-protein crosslink repair.
  5. In dividing cells, Wss1 is exclusively present in the mother cell.
    Title: The Saccharomyces cerevisiae Wss1 protein is only present in mother cells.
  6. Involement of WSS1 in the DNA damage response.
    Title: Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables SUMO binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables SUMO ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloendopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metalloendopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables metallopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metallopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in histone catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein autosumoylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein sumoylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein sumoylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in replication-born double-strand break repair via sister chromatid exchange IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in translesion synthesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translesion synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in fungal-type vacuole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear envelope IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
metalloendopeptidase WSS1
NP_012002.1
  • SUMO-ligase and SUMO-targeted metalloprotease; involved in DNA repair; removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; clears chromatin-bound sumoylated proteins; localizes to single spot on nuclear periphery of mother cells but not daughters; exhibits vacuolar localization upon genotoxic stress; activated by DNA binding; member of minigluzincins protease family with mammalian DVC1/Spartan

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001140.6 Reference assembly

    Range
    371749..372558
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179264.1 → NP_012002.1  TPA: metalloendopeptidase WSS1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012002.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DL83, P38838
    UniProtKB/TrEMBL
    B3LSP8, C7GTW1, C8Z9S9, N1P2U5
    Conserved Domains (1) summary
    pfam08325
    Location:27 → 221
    WLM; WLM domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38838.1

Gene LinkOut

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