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NPR2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856647, updated on 11-Sep-2024

Summary

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Official Symbol
NPR2
Primary source
SGD:S000000788
Locus tag
YEL062W
See related
AllianceGenome:SGD:S000000788; FungiDB:YEL062W; VEuPathDB:YEL062W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in several processes, including negative regulation of TORC1 signaling; positive regulation of cellular response to amino acid starvation; and positive regulation of peptidyl-serine phosphorylation. Is extrinsic component of fungal-type vacuolar membrane. Part of GATOR1 complex. Used to study cancer. Human ortholog(s) of this gene implicated in focal epilepsy. Orthologous to human NPRL2 (NPR2 like, GATOR1 complex subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: V
Exon count:
1
Sequence:
Chromosome: V; NC_001137.3 (34407..36254)

Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene Avt2p Neighboring gene arginine permease CAN1 Neighboring gene kinesin motor protein CIN8 Neighboring gene proteinase B

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill. Schenk PW, et al. Mol Pharmacol, 2003 Aug. PMID 12869630
  2. A second nitrogen permease regulator in Saccharomyces cerevisiae. Rousselet G, et al. FEBS Lett, 1995 Feb 13. PMID 7867803
  3. The SEACIT complex is involved in the maintenance of vacuole-mitochondria contact sites and controls mitophagy. Ma Y, et al. Cell Mol Life Sci, 2019 Apr. PMID 30673821, Free PMC Article
  4. Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Wu X, et al. Mol Biol Cell, 2011 Nov. PMID 21900499, Free PMC Article
  5. Isoflurane induces Art2-Rsp5-dependent endocytosis of Bap2 in yeast. Kozu F, et al. FEBS Open Bio, 2021 Nov. PMID 34536986, Free PMC Article

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These data revealed the metabolic basis by which the Npr2 complex regulates cellular homeostasis and demonstrated a key function for TORC1 in regulating the synthesis and utilization of glutamine as a nitrogen source.
    Title: Npr2 inhibits TORC1 to prevent inappropriate utilization of glutamine for biosynthesis of nitrogen-containing metabolites.
  2. Npr2-Npr3-dependent GTP hydrolysis of Gtr1 and direct binding of Gtr2 to Kog1, are involved in TORC1 inactivation and autophagic induction
    Title: Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy.
  3. A visual screen uncovered three regulators of autophagy-Iml1p, Npr2p, and Npr3p-which function in the same complex and are selectively required for non-nitrogen starvation-induced autophagy
    Title: Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation.
  4. Data indicate that Npr2, a homolog of human NPRL2, is a phosphorylation-dependent target of the SCF(Grr1) E3 ubiquitin ligase that plays a role in cell growth on some nitrogen sources.
    Title: Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources.
  5. Npr2/3 complex mediates an amino acid starvation signal to TORC1.
    Title: A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
contributes_to GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in cellular response to amino acid starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to nitrogen starvation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in cellular response to nitrogen starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of TORC1 signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of TORC1 signaling IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of TORC1 signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of TORC1 signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of autophagy IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of autophagy IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of autophagy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proline transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of autophagosome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to xenobiotic stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in urea transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of GATOR1 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of GATOR1 complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Seh1-associated complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in vacuolar membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
nitrogen permease regulating protein NPR2
NP_010852.4
  • Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001137.3 Reference assembly

    Range
    34407..36254
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178877.3 → NP_010852.4  TPA: nitrogen permease regulating protein NPR2 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010852.4

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DLI8, P39923, Q6B264
    UniProtKB/TrEMBL
    A6ZQN9, G2WCH2
    Conserved Domains (1) summary
    cl24021
    Location:9 → 490
    NPR3; Nitrogen Permease regulator of amino acid transport activity 3
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P39923.2

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