RIP1 ubiquinol--cytochrome-c reductase catalytic subunit RIP1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RIP1
Official Full Name: ubiquinol--cytochrome-c reductase catalytic subunit RIP1
Primary source: SGD:S000000750
Locus tag: YEL024W
See related: AllianceGenome:SGD:S000000750; FungiDB:YEL024W; VEuPathDB:YEL024W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ubiquinol-cytochrome-c reductase activity. Involved in mitochondrial electron transport, ubiquinol to cytochrome c. Located in mitochondrion. Part of respiratory chain complex III. Human ortholog(s) of this gene implicated in mitochondrial complex III deficiency. Orthologous to several human genes including UQCRFS1 (ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RIP1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (107260..107907)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study presents the structure of Saccharomyces cerevisiae Bcs1, an AAA-ATPase of the inner mitochondrial membrane. Bcs1 facilitates the translocation of the Rieske protein, Rip1, which requires folding and incorporation of a 2Fe-2S cluster before translocation and subsequent integration into the bc1 complex.
Title: Structure of the Bcs1 AAA-ATPase suggests an airlock-like translocation mechanism for folded proteins.
These findings suggest that Iba57p participates in maturation of the [2Fe-2S]-cluster into the Rieske protein and that Rieske protein plays important roles in the conformation and functionality of mitochondrial supercomplex III/IV in the electron transport chain.
Title: Iba57p participates in maturation of a [2Fe-2S]-cluster Rieske protein and in formation of supercomplexes III/IV of Saccharomyces cerevisiae electron transport chain.
findings show that the matrix protein Mzm1 functions as a Rip1 chaperone, stabilizing Rip1 prior to the translocation step
Title: Late-stage maturation of the Rieske Fe/S protein: Mzm1 stabilizes Rip1 but does not facilitate its translocation by the AAA ATPase Bcs1.
The oligomeric AAA-ATPase Bcs1 is involved in export of the folded Fe-S domain of Rip1 across the inner membrane and insertion of its transmembrane segment into an assembly intermediate of the cytochrome bc(1) complex.
Title: A pathway of protein translocation in mitochondria mediated by the AAA-ATPase Bcs1.
Mzm1 may function primarily to stabilize Rip1 prior to inner membrane (IM) insertion or alternatively to aid in the presentation of Rip1 to the inner membrane translocation complex for extrusion of the folded domain containing the iron-sulfur center.
Title: The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria.
It enhances methylmercury toxicity through production of reactive osygen species in budding yeast.
Title: Rip1 enhances methylmercury toxicity through production of reactive oxygen species (ROS) in budding yeast.
Cbp3p and Cbp4p are two mitochondrial proteins which are postulated to act as chaperones in bc(1) complex formation.
Title: Organization of assembly factors Cbp3p and Cbp4p and their effect on bc(1) complex assembly in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquinol-cytochrome-c reductase activity	IEA Inferred from Electronic Annotation more info
enables ubiquinol-cytochrome-c reductase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in aerobic respiration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial electron transport, ubiquinol to cytochrome c	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial electron transport, ubiquinol to cytochrome c	IDA Inferred from Direct Assay more info	PubMed
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IPI Inferred from Physical Interaction more info	PubMed
part_of respiratory chain complex III	IBA Inferred from Biological aspect of Ancestor more info
part_of respiratory chain complex III	IDA Inferred from Direct Assay more info	PubMed
part_of respiratory chain complex III	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: ubiquinol--cytochrome-c reductase catalytic subunit RIP1

NP_010890.3

EC 7.1.1.8

Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

107260..107907

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178839.3 → NP_010890.3 TPA: ubiquinol--cytochrome-c reductase catalytic subunit RIP1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010890.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLM4, P08067

UniProtKB/TrEMBL

A6ZQS4, B3LRZ1, B5VHB1, C7GX18, C8Z6X3, G2WCK7, N1P7D2

Conserved Domains (2) summary

cd03470
Location:93 → 214

Rieske_cytochrome_bc1; Iron-sulfur protein (ISP) component of the bc(1) complex family, Rieske domain; The Rieske domain is a [2Fe-2S] cluster binding domain involved in electron transfer. The bc(1) complex is a multisubunit enzyme found in many different organisms including ...

pfam02921
Location:32 → 87

UCR_TM; Ubiquinol cytochrome reductase transmembrane region