SEC3 GTP-Rho binding exocyst subunit SEC3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SEC3
Official Full Name: GTP-Rho binding exocyst subunit SEC3
Primary source: SGD:S000000810
Locus tag: YER008C
See related: AllianceGenome:SGD:S000000810; FungiDB:YER008C; VEuPathDB:YER008C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PSL1
Summary: Enables phosphatidylinositol-4,5-bisphosphate binding activity and small GTPase binding activity. Involved in several processes, including Golgi to plasma membrane transport; endoplasmic reticulum inheritance; and exocyst assembly. Located in cellular bud neck; cellular bud tip; and mating projection tip. Part of exocyst and incipient cellular bud site. Orthologous to human EXOC1 (exocyst complex component 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SEC3 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (167808..171818, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N-terminus of Sec3 is required for cell wall integrity in yeast.
Title: The N-terminus of Sec3 is required for cell wall integrity in yeast.
Blocking actin-dependent delivery of secretory vesicles in act1-3 cells results in loss of Sec3p from the purified complex. The results are consistent with a model in which Sec3p and Exo70p can function exclusively on the plasma membrane while the other subunits are brought to them on secretory vesicles.
Title: Two subunits of the exocyst, Sec3p and Exo70p, can function exclusively on the plasma membrane.
Data indicate structural details of the interaction of exocyst complex Sec3p and Exo70p with lipid bilayers containing phosphatidylinositol (4,5)-bisphosphate (PIP2) by molecular dynamics simulations.
Title: Membrane targeting of the yeast exocyst complex.
only the ectopically located Sec3p subunit is capable of recruiting secretory vesicles to mitochondria.
Title: The role of Sec3p in secretory vesicle targeting and exocyst complex assembly.
Residues Phe77, Ile115 and Leu131 of Sec3 bind to an extended hydrophobic surface formed around switch regions I and II of Rho1.
Title: Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.
The interactions of Sec3 with phospholipids and Cdc42 play important roles in exocytosis and polarized cell growth.
Title: Membrane association and functional regulation of Sec3 by phospholipids and Cdc42.
Assembly of the exocyst occurs when the first subset of subunits, delivered on vesicles, joins Sec3p and Exo70p on the plasma membrane. Exocyst assembly serves to both target and tether vesicles to sites of exocytosis.
Title: Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-4,5-bisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi to plasma membrane transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Golgi to plasma membrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endoplasmic reticulum inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocyst assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocyst localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	HDA more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
part_of exocyst	IBA Inferred from Biological aspect of Ancestor more info
part_of exocyst	IDA Inferred from Direct Assay more info	PubMed
part_of exocyst	IEA Inferred from Electronic Annotation more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in prospore membrane	HDA more info	PubMed

General protein information

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Preferred Names: GTP-Rho binding exocyst subunit SEC3

NP_010924.1

Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

167808..171818 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178899.1 → NP_010924.1 TPA: GTP-Rho binding exocyst subunit SEC3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010924.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLQ5, P33332

UniProtKB/TrEMBL

B3LS20, N1P5Q5

Conserved Domains (5) summary

PTZ00121
Location:283 → 478

PTZ00121; MAEBL; Provisional

cd13315
Location:82 → 232

PH_Sec3; Sec 3 Pleckstrin homology-like domain

pfam02463
Location:348 → 629

SMC_N; RecF/RecN/SMC N terminal domain

pfam15346
Location:358 → 444

ARGLU; Arginine and glutamate-rich 1

cl19950
Location:640 → 1316

Sec3_C; Exocyst complex component Sec3