OXA1 membrane insertase OXA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: OXA1
Official Full Name: membrane insertase OXA1
Primary source: SGD:S000000956
Locus tag: YER154W
See related: AllianceGenome:SGD:S000000956; FungiDB:YER154W; VEuPathDB:YER154W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables membrane insertase activity and mitochondrial ribosome binding activity. Involved in mitochondrial proton-transporting ATP synthase complex assembly; protein insertion into mitochondrial inner membrane; and protein insertion into mitochondrial inner membrane from matrix. Located in mitochondrial crista and mitochondrial inner boundary membrane. Orthologous to human OXA1L (OXA1L mitochondrial inner membrane protein). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See OXA1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (475020..476228)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes.
Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes.
The ER membrane complex (EMC) can functionally replace the Oxa1 insertase in mitochondria.
Title: The ER membrane complex (EMC) can functionally replace the Oxa1 insertase in mitochondria.
Data indicate that Escherichia coli Ffh protein bound to the large subunit of mitochondrial ribosomes, and could relieve, to some degree, the defect of Saccharomyces cerevisiae Oxa1 mutants.
Title: Partial suppression of Oxa1 mutants by mitochondria-targeted signal recognition particle provides insights into the evolution of the cotranslational insertion systems.
observations suggest that the function of Oxa1 is relevant not only for the biogenesis of conserved mitochondrial components such as respiratory chain complexes or ABC transporters but also for mitochondria-specific membrane proteins of eukaryotic origin
Title: The membrane insertase Oxa1 is required for efficient import of carrier proteins into mitochondria.
the close proximity of Oxa1 to ribosomes is not only used to improve membrane insertion but is also critical for the productive assembly of the subunits of the cytochrome c oxidase.
Title: Oxa1-ribosome complexes coordinate the assembly of cytochrome C oxidase in mitochondria.
Oxa1 behaves as a pore-forming translocase that is regulated in a membrane potential and substrate-dependent manner
Title: The mitochondrial oxidase assembly protein1 (Oxa1) insertase forms a membrane pore in lipid bilayers.
Findings show that the distribution of Oxa1 within the inner membrane is dynamic and adapts to different physiological needs.
Title: The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates.
neither ATP2 nor OXA1 mRNA targeting was affected by a block in translation initiation, indicating that translation may not be essential for mRNA anchoring
Title: Localization of mRNAs coding for mitochondrial proteins in the yeast Saccharomyces cerevisiae.
MrpL40, a large ribosomal subunit component, is physically interact with Oxa1.
Title: Mapping of the Saccharomyces cerevisiae Oxa1-mitochondrial ribosome interface and identification of MrpL40, a ribosomal protein in close proximity to Oxa1 and critical for oxidative phosphorylation complex assembly.
Yme1 probably chaperones the folding and/or assembly of Oxa1-exported Cox2 in the absence of Mrg1 or Mgr3, since respiratory growth and cytochrome c oxidase assembly in a cox18 mgr3 double-mutant strain overexpressing OXA1 is YME1 dependent
Title: Translocation and assembly of mitochondrially coded Saccharomyces cerevisiae cytochrome c oxidase subunit Cox2 by Oxa1 and Yme1 in the absence of Cox18.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables membrane insertase activity	IBA Inferred from Biological aspect of Ancestor more info
enables membrane insertase activity	IEA Inferred from Electronic Annotation more info
enables membrane insertase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mitochondrial ribosome binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial proton-transporting ATP synthase complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein insertion into mitochondrial inner membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein insertion into mitochondrial inner membrane from matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein insertion into mitochondrial inner membrane from matrix	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial crista	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner boundary membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: membrane insertase OXA1

NP_011081.1

Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

475020..476228

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179044.1 → NP_011081.1 TPA: membrane insertase OXA1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011081.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM61, P39952

UniProtKB/TrEMBL

A6ZRB1, B3LRR7, C7GQ42, C8Z7H7, G2WD41, N1PA62

Conserved Domains (1) summary

cd20069
Location:125 → 319

5TM_Oxa1-like; Five transmembrane core domain of mitochondrial inner membrane protein Oxa1 and similar proteins