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nfsA NADPH-dependent nitro/quinone reductase NfsA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945483, updated on 19-Sep-2024

Summary

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Official Symbol
nfsA
Official Full Name
NADPH-dependent nitro/quinone reductase NfsA
Primary source
ECOCYC:EG11261
Locus tag
b0851
See related
ASAP:ABE-0002900
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0842; mda18; mdaA; ybjB
Summary
NfsA overexpression results in resistance to cytotoxic drugs includingAdriamycin and the tumoricidal agent DMP 840. nfsA is co-transcribed with ybjC. nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG11261]. The nfsA gene encodes the major oxygen-insensitive nitroreductase present in Escherichia coli. [More information is available at EcoCyc: EG11261].
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Genomic context

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Sequence:
NC_000913.3 (891184..891906)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative inner membrane protein Neighboring gene reduced glutaredoxin 1 Neighboring gene DUF1418 domain-containing protein YbjC Neighboring gene ribosomal protein S6 modification protein Neighboring gene protein YbjN

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN. Day MA, et al. Biochem J, 2021 Jul 16. PMID 34142705, Free PMC Article
  2. Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution. Zenno S, et al. J Bacteriol, 1998 Jan. PMID 9440535, Free PMC Article
  3. Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli. Kobori T, et al. Acta Crystallogr D Biol Crystallogr, 1999 Nov. PMID 10531489
  4. Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds: alteration of pyridine nucleotide binding by a single amino acid substitution. Kobori T, et al. J Biol Chem, 2001 Jan 26. PMID 11034992
  5. Vibrio harveyi nitroreductase is also a chromate reductase. Kwak YH, et al. Appl Environ Microbiol, 2003 Aug. PMID 12902220, Free PMC Article

See all (39) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
    Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
  2. The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
    Title: The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
  3. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
    Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
  4. reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism
    Title: Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity.
  5. Findings revealed that Phe42 is important for maintaining NfsA activity and structure.
    Title: Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA.
  6. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
    Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables FMN binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables NAD(P)H dehydrogenase (quinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables chromate reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
NADPH-dependent nitro/quinone reductase NfsA
NP_415372.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    891184..891906
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415372.1 NADPH-dependent nitro/quinone reductase NfsA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415372.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7A7AXF1
    Conserved Domains (1) summary
    PRK10765
    Location:1 → 240
    PRK10765; oxygen-insensitive NADPH nitroreductase
Nucleotide Protein
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