U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

cheY chemotaxis protein CheY [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946393, updated on 19-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
cheY
Official Full Name
chemotaxis protein CheY
Primary source
ECOCYC:EG10150
Locus tag
b1882
See related
ASAP:ABE-0006280
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1883
Summary
CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs). [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA(L)-his-P protein. [More information is available at EcoCyc: EG10150].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (1967048..1967437, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene flagellar biosynthesis protein FlhA Neighboring gene flagellar biosynthesis protein FlhB Neighboring gene chemotaxis protein CheZ Neighboring gene protein-glutamate methylesterase/protein glutamine deamidase Neighboring gene chemotaxis protein methyltransferase

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli. Zhu X, et al. J Bacteriol, 1996 Jul. PMID 8763950, Free PMC Article
  2. The nonequilibrium mechanism for ultrasensitivity in a biological switch: sensing by Maxwell's demons. Tu Y. Proc Natl Acad Sci U S A, 2008 Aug 19. PMID 18687900, Free PMC Article
  3. Kinetic traps in the folding of beta alpha-repeat proteins: CheY initially misfolds before accessing the native conformation. Kathuria SV, et al. J Mol Biol, 2008 Oct 3. PMID 18619461
  4. Two variable active site residues modulate response regulator phosphoryl group stability. Thomas SA, et al. Mol Microbiol, 2008 Jul. PMID 18557815, Free PMC Article
  5. Interactions between chemotaxis genes and flagellar genes in Escherichia coli. Parkinson JS, et al. J Bacteriol, 1983 Jul. PMID 6305913, Free PMC Article

See all (161) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
    Title: The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
  2. Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
    Title: Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
  3. Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli.
    Title: Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli.
  4. Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.
    Title: Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.
  5. The suitability for FRET analysis as well as functionality of fusions with SNAP- and HaloTag could be demonstrated by studying the transient interaction between chemotaxis response regulator CheY and its phosphatase CheZ
    Title: A gateway-based system for fast evaluation of protein-protein interactions in bacteria.
  6. study identified K91 and K109 as the major sites whose acetylation level in vivo increases in response to acetate
    Title: CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.
  7. the enhanced reactivity of CheY DR reflected partial acquisition of catalytic and structural features of HAD phosphatases.
    Title: Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.
  8. Authors observe an apparent decrease and redistribution of mus-ms dynamics of allosteric response upon phosphorylation (and accompanying Mg(2+) saturation) of CheY.
    Title: Colocalization of fast and slow timescale dynamics in the allosteric signaling protein CheY.
  9. The flagellar motor adapts to changes in steady-state level of chemotaxis response regulator CheY-P by adjusting the number of FliM molecules to which CheY-P binds. Extreme motor ultrasensitivity broadens our understanding of allostery mechanisms
    Title: Ultrasensitivity of an adaptive bacterial motor.
  10. Amino acid substitutions distant from the active site can affect CheZ catalytic activity and CheYp binding, perhaps via the propagation of structural or dynamic perturbations through a helical bundle.
    Title: Action at a distance: amino acid substitutions that affect binding of the phosphorylated CheY response regulator and catalysis of dephosphorylation can be far from the CheZ phosphatase active site.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acetyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphorelay response regulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within aerotaxis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in archaeal or bacterial-type flagellum-dependent cell motility IEA
Inferred from Electronic Annotation
more info
  
involved_in bacterial-type flagellum-dependent swimming motility IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in bacterial-type flagellum-dependent swimming motility NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in chemotaxis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within chemotaxis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chemotaxis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in internal peptidyl-lysine acetylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phosphorelay signal transduction system IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in phosphorelay signal transduction system IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within regulation of bacterial-type flagellum-dependent cell motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of bacterial-type flagellum-dependent cell motility NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of chemotaxis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of chemotaxis NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within thermotaxis IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in bacterial-type flagellum NAS
Non-traceable Author Statement
more info
 PubMed 
located_in bacterial-type flagellum basal body, C ring NAS
Non-traceable Author Statement
more info
 PubMed 
located_in bacterial-type flagellum rotor complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
chemotaxis protein CheY

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1967048..1967437 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416396.1 chemotaxis protein CheY [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416396.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AJ61
    Conserved Domains (1) summary
    PRK10610
    Location:1 → 129
    PRK10610; chemotaxis protein CheY
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.