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    YSP2 Ysp2p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851926, updated on 28-Oct-2024

    Summary

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    Official Symbol
    YSP2
    Official Full Name
    Ysp2p
    Primary source
    SGD:S000002734
    Locus tag
    YDR326C
    See related
    AllianceGenome:SGD:S000002734; FungiDB:YDR326C; VEuPathDB:YDR326C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    LAM2; LTC4
    Summary
    Enables sterol binding activity and sterol transfer activity. Involved in intracellular sterol transport. Acts upstream of or within sterol transport. Located in cortical endoplasmic reticulum and mitochondrion. Orthologous to human GRAMD1A (GRAM domain containing 1A) and GRAMD1C (GRAM domain containing 1C). [provided by Alliance of Genome Resources, Oct 2024]
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    Genomic context

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    See YSP2 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1120609..1124925, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Utp4p Neighboring gene condensin subunit YCG1 Neighboring gene SCF ubiquitin ligase subunit SKP1 Neighboring gene Pex3p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mitochondrial matrix fragmentation as a protection mechanism of yeast Saccharomyces cerevisiae. Knorre DA, et al. Biochemistry (Mosc), 2008 Nov. PMID 19120030
    2. Ysp2 mediates death of yeast induced by amiodarone or intracellular acidification. Sokolov S, et al. Biochim Biophys Acta, 2006 Sep-Oct. PMID 16962064
    3. Molecular basis for sterol transport by StART-like lipid transfer domains. Horenkamp FA, et al. EMBO J, 2018 Mar 15. PMID 29467216, Free PMC Article
    4. Proteomic analysis reveals complex metabolic regulation in Saccharomyces cerevisiae cells against multiple inhibitors stress. Lv YJ, et al. Appl Microbiol Biotechnol, 2014 Mar. PMID 24442506
    5. Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites. Tong J, et al. Proc Natl Acad Sci U S A, 2018 Jan 30. PMID 29339490, Free PMC Article

    See all (56) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the beta-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
      Title: TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the β-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
    2. Ltc1 and Ltc3/4 function at the vacuole and plasma membrane, respectively, to create membrane domains that partition upstream regulators of the TORC1 and TORC2 signaling pathways to coordinate cellular stress responses with sterol homeostasis.
      Title: Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.
    3. Ysp2p function correlates with sterol binding to its StART-like domain.
      Title: A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport.
    4. Intracellular acidification results in the protonation of superoxide anion (O2-*) to form HO2, one of the most aggressive Reactive oxygen species, which in turn induces Ysp2-mediated programmed cell death.
      Title: Ysp2 mediates death of yeast induced by amiodarone or intracellular acidification.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables sterol binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sterol binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sterol transfer activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sterol transfer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intracellular sterol transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular sterol transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within sterol transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell periphery HDA PubMed 
    is_active_in cortical endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cortical endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum-plasma membrane contact site IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrial membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in organelle IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    Ysp2p
    NP_010613.1
    • Sterol-binding protein located at plasma membrane-ER contact sites; role in retrograde transport of sterols from plasma membrane to ER, which is inhibited via phosphorylation by TORC2-dependent protein kinase Ypk1p; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1120609..1124925 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180634.1 → NP_010613.1  TPA: Ysp2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010613.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSV9, Q06681
      UniProtKB/TrEMBL
      A6ZYS9, C8Z5N9, N1P8N1
      Conserved Domains (2) summary
      cd13220
      Location:660 → 753
      PH-GRAM_GRAMDC; GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain
      pfam16016
      Location:854 → 998
      DUF4782; Domain of unknown function (DUF4782)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q06681.1

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