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    Mid1 midline 1 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 54252, updated on 10-Oct-2024

    Summary

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    Official Symbol
    Mid1provided by RGD
    Official Full Name
    midline 1provided by RGD
    Primary source
    RGD:2640
    See related
    EnsemblRapid:ENSRNOG00000003613 AllianceGenome:RGD:2640
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Fxy; Midline1
    Summary
    Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and ubiquitin protein ligase binding activity. Predicted to be involved in positive regulation of stress-activated MAPK cascade and protein localization to microtubule. Predicted to act upstream of or within negative regulation of microtubule depolymerization. Predicted to be located in cytoplasm and microtubule. Predicted to colocalize with cytoplasmic microtubule. Human ortholog(s) of this gene implicated in Opitz GBBB syndrome type I. Orthologous to human MID1 (midline 1). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Muscle (RPKM 71.3), Kidney (RPKM 61.8) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Mid1 in Genome Data Viewer
    Location:
    Xq13
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) X NC_086039.1 (27678248..28053049, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) X NC_051356.1 (24116674..24491205, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) X NC_005120.4 (25458782..25839941, complement)

    Chromosome X - NC_086039.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC120099331 Neighboring gene chloride voltage-gated channel 4 Neighboring gene uncharacterized LOC134484072 Neighboring gene capping actin protein of muscle Z-line subunit alpha 1, pseudogene 1 Neighboring gene Rho GTPase activating protein 6 Neighboring gene holocytochrome c synthase Neighboring gene amelogenin, X-linked

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1. Winter J, et al. BMC Mol Biol, 2007 Nov 15. PMID 18005432, Free PMC Article
    2. Evolutionary rate of a gene affected by chromosomal position. Perry J, et al. Curr Biol, 1999 Sep 9. PMID 10508587
    3. Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A. Aranda-Orgillés B, et al. PLoS One, 2008. PMID 18949047, Free PMC Article
    4. Widely spaced alternative promoters, conserved between human and rodent, control expression of the Opitz syndrome gene MID1. Landry JR, et al. Genomics, 2002 Nov. PMID 12408967
    5. Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules. Berti C, et al. BMC Cell Biol, 2004 Feb 29. PMID 15070402, Free PMC Article

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables microtubule binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables phosphoprotein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within negative regulation of microtubule depolymerization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of stress-activated MAPK cascade ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of microtubule cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in centriolar satellite IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in centriolar satellite ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasmic microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in microtubule IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in microtubule cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase Midline-1
    Names
    Finger on X and Y (in rat only on X)
    RING finger protein Midline-1
    RING-type E3 ubiquitin transferase Midline-1
    midline 1 (Opitz/BBB syndrome)
    tripartite motif-containing protein 18
    NP_075216.1
    XP_006256887.1
    XP_017457631.1
    XP_017457632.1
    XP_038955925.1
    XP_038955926.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001431605.1 → NP_001418534.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/Swiss-Prot
      P82458
      UniProtKB/TrEMBL
      A6K2D8

    2. NM_001431606.1 → NP_001418535.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/Swiss-Prot
      P82458
      UniProtKB/TrEMBL
      A6K2D8

    3. NM_001431607.1 → NP_001418536.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/Swiss-Prot
      P82458
      UniProtKB/TrEMBL
      A6K2D8

    4. NM_001431608.1 → NP_001418537.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/Swiss-Prot
      P82458
      UniProtKB/TrEMBL
      A6K2D8

    5. NM_001431609.1 → NP_001418538.1  E3 ubiquitin-protein ligase Midline-1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021

    6. NM_022927.2 → NP_075216.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

      See identical proteins and their annotated locations for NP_075216.1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000021
      UniProtKB/Swiss-Prot
      P82458
      UniProtKB/TrEMBL
      A6K2D8
      Related
      ENSRNOP00000004873.5, ENSRNOT00000004873.8
      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19822
      Location:168 → 214
      Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:485 → 661
      SPRY; SPRY domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086039.1 Reference GRCr8

      Range
      27678248..28053049 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017602142.3 → XP_017457631.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19822
      Location:168 → 214
      Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:485 → 661
      SPRY; SPRY domain

    2. XM_039099997.2 → XP_038955925.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19822
      Location:168 → 214
      Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:485 → 661
      SPRY; SPRY domain

    3. XM_017602143.3 → XP_017457632.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19822
      Location:168 → 214
      Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:485 → 661
      SPRY; SPRY domain

    4. XM_039099998.1 → XP_038955926.1  E3 ubiquitin-protein ligase Midline-1 isoform X2

      Related
      ENSRNOP00000101804.1, ENSRNOT00000141079.1
      Conserved Domains (6) summary
      smart00502
      Location:182 → 306
      BBC; B-Box C-terminal domain
      cd00063
      Location:343 → 443
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:285 → 336
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:447 → 623
      SPRY; SPRY domain

    5. XM_006256825.5 → XP_006256887.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd16753
      Location:8 → 61
      RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
      cd19822
      Location:168 → 214
      Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
      cd19836
      Location:115 → 164
      Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cl02614
      Location:485 → 661
      SPRY; SPRY domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P82458.1 GenPept UniProtKB/Swiss-Prot:P82458

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