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    MSLN mesothelin [ Homo sapiens (human) ]

    Gene ID: 10232, updated on 2-Nov-2024

    Summary

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    Official Symbol
    MSLNprovided by HGNC
    Official Full Name
    mesothelinprovided by HGNC
    Primary source
    HGNC:HGNC:7371
    See related
    Ensembl:ENSG00000102854 MIM:601051; AllianceGenome:HGNC:7371
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MPF; SMRP
    Summary
    This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
    Expression
    Biased expression in lung (RPKM 16.4), stomach (RPKM 4.7) and 7 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MSLN in Genome Data Viewer
    Location:
    16p13.3
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 16 NC_000016.10 (760734..768865)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 16 NC_060940.1 (764710..773383)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 16 NC_000016.9 (810734..818865)

    Chromosome 16 - NC_000016.10Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6949 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6950 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6951 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6952 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6953 Neighboring gene hydroxyacylglutathione hydrolase like Neighboring gene cytosolic iron-sulfur assembly component 3 Neighboring gene Sharpr-MPRA regulatory region 977 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:790544-791139 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6956 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr16:800614-801181 Neighboring gene MSLN promoter region Neighboring gene mesothelin like Neighboring gene microRNA 662 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:836847-837654 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6957 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6958 Neighboring gene RNA pseudouridine synthase domain containing 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Mesothelin binding to CA125/MUC16 promotes pancreatic cancer cell motility and invasion via MMP-7 activation. Chen SH, et al. Sci Rep, 2013. PMID 23694968, Free PMC Article
    2. Mesothelin confers pancreatic cancer cell resistance to TNF-α-induced apoptosis through Akt/PI3K/NF-κB activation and IL-6/Mcl-1 overexpression. Bharadwaj U, et al. Mol Cancer, 2011 Aug 31. PMID 21880146, Free PMC Article
    3. Serum mesothelin and megakaryocyte potentiating factor in pancreatic and biliary cancers. Sharon E, et al. Clin Chem Lab Med, 2012 Apr. PMID 22149739, Free PMC Article
    4. Tissue and serum mesothelin are potential markers of neoplastic progression in Barrett's associated esophageal adenocarcinoma. Rizk NP, et al. Cancer Epidemiol Biomarkers Prev, 2012 Mar. PMID 22237988, Free PMC Article
    5. Mesothelin-targeted agents in clinical trials and in preclinical development. Kelly RJ, et al. Mol Cancer Ther, 2012 Mar. PMID 22351743, Free PMC Article

    See all (178) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mesothelin-based CAR-T cells exhibit potent antitumor activity against ovarian cancer.
      Title: Mesothelin-based CAR-T cells exhibit potent antitumor activity against ovarian cancer.
    2. Mesothelin expression in gynecologic carcinosarcoma: clinicopathological significance and correlation with HER2 expression.
      Title: Mesothelin expression in gynecologic carcinosarcoma: clinicopathological significance and correlation with HER2 expression.
    3. Enhanced Expression of Glycolytic Enzymes and Succinate Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute Myeloid Leukemia.
      Title: Enhanced Expression of Glycolytic Enzymes and Succinate Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute Myeloid Leukemia.
    4. Tumor resistance to anti-mesothelin CAR-T cells caused by binding to shed mesothelin is overcome by targeting a juxtamembrane epitope.
      Title: Tumor resistance to anti-mesothelin CAR-T cells caused by binding to shed mesothelin is overcome by targeting a juxtamembrane epitope.
    5. Novel mesothelin-targeted chimeric antigen receptor-modified UNKT cells are highly effective in inhibiting tumor progression.
      Title: Novel mesothelin-targeted chimeric antigen receptor-modified UNKT cells are highly effective in inhibiting tumor progression.
    6. High mesothelin expression by immunohistochemistry predicts improved survival in pleural mesothelioma.
      Title: High mesothelin expression by immunohistochemistry predicts improved survival in pleural mesothelioma.
    7. Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma.
      Title: Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma.
    8. Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer.
      Title: Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer.
    9. Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.
      Title: Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.
    10. Host Mesothelin Expression Increases Ovarian Cancer Metastasis in the Peritoneal Microenvironment.
      Title: Host Mesothelin Expression Increases Ovarian Cancer Metastasis in the Peritoneal Microenvironment.
    Submit: New GeneRIF Correction See all GeneRIFs (150)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell adhesion NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell-matrix adhesion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cell surface IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum lumen TAS
    Traceable Author Statement
    more info
    		  
    located_in extracellular region TAS
    Traceable Author Statement
    more info
    		  
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in side of membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mesothelin
    Names
    CAK1 antigen
    megakaryocyte potentiating factor
    pre-pro-megakaryocyte-potentiating factor
    soluble MPF mesothelin related protein

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001177355.3 → NP_001170826.1  mesothelin isoform 1 preproprotein

      See identical proteins and their annotated locations for NP_001170826.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).
      Source sequence(s)
      AL031258, AY743922, BC003512, BC009272
      Consensus CDS
      CCDS45370.1
      UniProtKB/Swiss-Prot
      Q13421
      Related
      ENSP00000456008.1, ENST00000563941.5
      Conserved Domains (1) summary
      pfam06060
      Location:1 → 608
      Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

    2. NM_005823.6 → NP_005814.2  mesothelin isoform 1 preproprotein

      See identical proteins and their annotated locations for NP_005814.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1).
      Source sequence(s)
      AL031258, AY743922, BC003512
      Consensus CDS
      CCDS45370.1
      UniProtKB/Swiss-Prot
      Q13421
      Related
      ENSP00000442965.2, ENST00000545450.7
      Conserved Domains (1) summary
      pfam06060
      Location:1 → 608
      Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

    3. NM_013404.4 → NP_037536.2  mesothelin isoform 2 preproprotein

      See identical proteins and their annotated locations for NP_037536.2

      Status: REVIEWED

      Description
      Transcript Variant: This transcript variant (2) uses an alternate splice site in the coding region, compared to variant 1. Variant 2 encodes isoform 2 which is longer than isoform 1.
      Source sequence(s)
      AL545401, BC003512, BC009272, CB266931, D49441
      Consensus CDS
      CCDS32356.1
      UniProtKB/Swiss-Prot
      D3DU65, Q13421, Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2, Q9UCB2, Q9UK57
      Related
      ENSP00000372313.3, ENST00000382862.7
      Conserved Domains (1) summary
      pfam06060
      Location:1 → 616
      Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000016.10 Reference GRCh38.p14 Primary Assembly

      Range
      760734..768865
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060940.1 Alternate T2T-CHM13v2.0

      Range
      764710..773383
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q13421.2 GenPept UniProtKB/Swiss-Prot:Q13421

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