Mars1 methionine-tRNA synthetase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mars1provided by MGI
Official Full Name: methionine-tRNA synthetase 1provided by MGI
Primary source: MGI:MGI:1345633
See related: Ensembl:ENSMUSG00000040354 AllianceGenome:MGI:1345633
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mars; Metrs; Mtrns
Summary: The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010]
Expression: Ubiquitous expression in adrenal adult (RPKM 23.2), ovary adult (RPKM 19.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D3; 10 74.5 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (127132090..127147655, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (127296221..127311786, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

X67083 (e-PCR)
- UniSTS:160053

NoName (e-PCR)
- UniSTS:225335

PMC186318P1 (e-PCR)
- UniSTS:271695

PMC150726P4 (e-PCR)
- UniSTS:271076

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables methionine-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables methionine-tRNA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables methionine-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables methionine-tRNA ligase activity	ISS Inferred from Sequence or Structural Similarity more info
enables tRNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to epidermal growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to platelet-derived growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in methionyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in methionyl-tRNA aminoacylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within methionyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in methionyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within rRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in rRNA transcription	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of aminoacyl-tRNA synthetase multienzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of aminoacyl-tRNA synthetase multienzyme complex	IDA Inferred from Direct Assay more info	PubMed
part_of aminoacyl-tRNA synthetase multienzyme complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
located_in nucleolus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: methionine--tRNA ligase, cytoplasmic

Names: methionine tRNA ligase; methionyl-tRNA synthetase, cytoplasmic

NP_001003913.1

EC 6.1.1.10

NP_001165053.1

EC 6.1.1.10

XP_006513631.1

EC 6.1.1.10

XP_036011653.1

EC 6.1.1.10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001003913.2 → NP_001003913.1 methionine--tRNA ligase, cytoplasmic isoform 2

See identical proteins and their annotated locations for NP_001003913.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1.

Source sequence(s)

AC114678, BC079643, CJ278192

Consensus CDS

CCDS24237.1

UniProtKB/Swiss-Prot

Q68FL6

Related

ENSMUSP00000037446.6, ENSMUST00000037290.12

Conserved Domains (7) summary

cd07957
Location:644 → 773

Anticodon_Ia_Met; Anticodon-binding domain of methionyl tRNA synthetases

cd00814
Location:267 → 635

MetRS_core; catalytic core domain of methioninyl-tRNA synthetases

cd10307
Location:77 → 179

GST_C_MetRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of Methionyl-tRNA synthetase from higher eukaryotes

COG0625
Location:47 → 189

GstA; Glutathione S-transferase [Posttranslational modification, protein turnover, chaperones]

PRK12268
Location:266 → 821

PRK12268; methionyl-tRNA synthetase; Reviewed

cd00939
Location:847 → 891

MetRS_RNA; MetRS_RNA binding domain. This short RNA-binding domain is found at the C-terminus of MetRS in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is repeated in Drosophila MetRS. This domain consists of a helix-turn-helix structure, which is ...

cl00388
Location:1 → 68

Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold
NM_001171582.1 → NP_001165053.1 methionine--tRNA ligase, cytoplasmic isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AA510607, AC114678, BC079643, CJ278192

Consensus CDS

CCDS48716.1

UniProtKB/TrEMBL

E9QB02

Related

ENSMUSP00000130666.2, ENSMUST00000171564.8

Conserved Domains (7) summary

cd07957
Location:644 → 773

Anticodon_Ia_Met; Anticodon-binding domain of methionyl tRNA synthetases

cd00814
Location:267 → 635

MetRS_core; catalytic core domain of methioninyl-tRNA synthetases

cd10307
Location:77 → 179

GST_C_MetRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of Methionyl-tRNA synthetase from higher eukaryotes

COG0625
Location:47 → 189

GstA; Glutathione S-transferase [Posttranslational modification, protein turnover, chaperones]

PRK12268
Location:266 → 821

PRK12268; methionyl-tRNA synthetase; Reviewed

cd00939
Location:855 → 899

MetRS_RNA; MetRS_RNA binding domain. This short RNA-binding domain is found at the C-terminus of MetRS in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is repeated in Drosophila MetRS. This domain consists of a helix-turn-helix structure, which is ...

cl00388
Location:1 → 68

Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

127132090..127147655 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155760.1 → XP_036011653.1 methionine--tRNA ligase, cytoplasmic isoform X2

Conserved Domains (1) summary

PLN02610
Location:1 → 554

PLN02610; probable methionyl-tRNA synthetase
XM_006513568.5 → XP_006513631.1 methionine--tRNA ligase, cytoplasmic isoform X1

Conserved Domains (2) summary

PLN02610
Location:1 → 524

PLN02610; probable methionyl-tRNA synthetase

cd00939
Location:531 → 575

MetRS_RNA; MetRS_RNA binding domain. This short RNA-binding domain is found at the C-terminus of MetRS in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is repeated in Drosophila MetRS. This domain consists of a helix-turn-helix structure, which is ...