MCRS1 microspherule protein 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: MCRS1provided by HGNC
Official Full Name: microspherule protein 1provided by HGNC
Primary source: HGNC:HGNC:6960
See related: Ensembl:ENSG00000187778 MIM:609504; AllianceGenome:HGNC:6960
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: P78; MCRS2; MSP58; INO80Q; ICP22BP
Summary: Enables RNA binding activity and telomerase inhibitor activity. Involved in several processes, including chromatin remodeling; positive regulation of protein localization to nucleolus; and regulation of nucleobase-containing compound metabolic process. Located in cytoplasm; nucleolus; and nucleoplasm. Part of Ino80 complex; MLL1 complex; and NSL complex. [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in heart (RPKM 7.8), thyroid (RPKM 7.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 12q13.12

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (49558299..49568142, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (49520555..49530398, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (49952082..49961925, complement)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The molecular characteristics and functional roles of microspherule protein 1 (MCRS1) in gene expression, cell proliferation, and organismic development.
Title: The molecular characteristics and functional roles of microspherule protein 1 (MCRS1) in gene expression, cell proliferation, and organismic development.
MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles.
Title: MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles.
This study confirmed the specific tumor suppressive activity of MCRS1 in gastric cancer proliferation, invasion and migration and suggested that it might inhibit the progression of gastric cancer through its interaction with Pkmyt1.
Title: Effects of MCRS1 on proliferation, migration, invasion, and epithelial mesenchymal transition of gastric cancer cells by interacting with Pkmyt1 protein kinase.
Our data suggest that decreased expression of miR-186 in hepatocellular carcinoma is responsible for the high expression of MCRS1
Title: miR-186 modulates hepatocellular carcinoma cell proliferation and mobility via targeting MCRS1-mediated Wnt/β-catenin signaling.
Authors show that Mps1 regulates chromosome alignment through MCRS1, a spindle assembly factor that controls the dynamics of the minus end of kinetochore microtubules. Mps1 binds and phosphorylates MCRS1.
Title: Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.
Study shows that MCRS1 binds to cytoplasmic dynein and contributes to the establishment of centriolar satellites and ciliogenesis.
Title: MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.
MSP58 downregulation suppressed the proliferation and invasion of renal cell carcinoma cells.
Title: Knockdown of MSP58 inhibits the proliferation and metastasis in human renal cell carcinoma cells.
MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36.
Title: Aurora-A regulates MCRS1 function during mitosis.
RINT-1 interacts with MSP58 and UBF within nucleoli and plays a role in ribosomal gene transcription.
Title: RINT-1 interacts with MSP58 within nucleoli and plays a role in ribosomal gene transcription.
The immunoreactivity score (IRS) of MSP58 increased with tumor grade with grade IV gliomas exhibiting the highest expression and showed a highly significant positive correlation with the Ki-67 index (r = 0.65, P < 0.001).
Title: Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS-F09935 (e-PCR)
- UniSTS:26162

STS-AA099057 (e-PCR)
- UniSTS:2898

RH77809 (e-PCR)
- UniSTS:42066

MCRS1_9110 (e-PCR)
- UniSTS:468082

RH41888 (e-PCR)
- UniSTS:72695

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables G-quadruplex RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables poly(G) binding	IDA Inferred from Direct Assay more info	PubMed
enables poly(U) RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables telomerase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of telomere maintenance via telomere lengthening	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of protein localization to nucleolus	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of telomere maintenance in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein modification process	TAS Traceable Author Statement more info	PubMed
involved_in regulation of DNA repair	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA strand elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of embryonic development	ISO Inferred from Sequence Orthology more info
involved_in telomere maintenance	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of Ino80 complex	IDA Inferred from Direct Assay more info	PubMed
part_of MLL1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of NSL complex	IBA Inferred from Biological aspect of Ancestor more info
part_of NSL complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISS Inferred from Sequence or Structural Similarity more info
part_of histone acetyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: microspherule protein 1

Names: 58 kDa microspherule protein; INO80 complex subunit J; INO80 complex subunit Q; cell cycle-regulated factor (78 kDa); cell cycle-regulated factor p78

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001012300.1 → NP_001012300.1 microspherule protein 1 isoform 2

See identical proteins and their annotated locations for NP_001012300.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) encodes the longest isoform (2).

Source sequence(s)

AY336730, BC011794

Consensus CDS

CCDS31795.1

UniProtKB/Swiss-Prot

Q96EZ8

Related

ENSP00000349640.4, ENST00000357123.8

Conserved Domains (2) summary

cd00060
Location:358 → 458

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam13325
Location:147 → 344

MCRS_N; N-terminal region of micro-spherule protein
NM_001278341.2 → NP_001265270.1 microspherule protein 1 isoform 3

See identical proteins and their annotated locations for NP_001265270.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon, compared to variant 2. The resulting isoform (3) has a shorter N-terminus, compared to isoform 2.

Source sequence(s)

BC011794, BX538079, HY078747, R66413

Consensus CDS

CCDS61118.1

Related

ENSP00000444982.1, ENST00000546244.5

Conserved Domains (2) summary

cd00060
Location:154 → 254

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam13325
Location:1 → 140

MCRS_N; N-terminal region of micro-spherule protein
NM_006337.5 → NP_006328.2 microspherule protein 1 isoform 1

See identical proteins and their annotated locations for NP_006328.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) has an alternate 5' coding sequence, compared to variant 2. The resulting isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2.

Source sequence(s)

BC011794, HY078747, R66413

Consensus CDS

CCDS8787.1

UniProtKB/Swiss-Prot

O14742, O75497, Q6VN53, Q7Z372, Q96EZ8

Related

ENSP00000345358.4, ENST00000343810.9

Conserved Domains (2) summary

cd00060
Location:345 → 445

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam13325
Location:134 → 331

MCRS_N; N-terminal region of micro-spherule protein

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000012.12 Reference GRCh38.p14 Primary Assembly

Range

49558299..49568142 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011537760.4 → XP_011536062.2 microspherule protein 1 isoform X1
XM_005268572.3 → XP_005268629.1 microspherule protein 1 isoform X3

See identical proteins and their annotated locations for XP_005268629.1

UniProtKB/Swiss-Prot

O14742, O75497, Q6VN53, Q7Z372, Q96EZ8

Related

ENSP00000448056.1, ENST00000550165.5

Conserved Domains (2) summary

cd00060
Location:345 → 445

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam13325
Location:134 → 331

MCRS_N; N-terminal region of micro-spherule protein
XM_017018690.2 → XP_016874179.1 microspherule protein 1 isoform X2

UniProtKB/Swiss-Prot

Q96EZ8

Related

ENST00000551625.5

Conserved Domains (2) summary

cd00060
Location:358 → 458

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam13325
Location:147 → 344

MCRS_N; N-terminal region of micro-spherule protein
XM_047428083.1 → XP_047284039.1 microspherule protein 1 isoform X4

Related

ENSP00000447514.1, ENST00000553173.5