Marchf1 membrane associated ring-CH-type finger 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Marchf1provided by MGI
Official Full Name: membrane associated ring-CH-type finger 1provided by MGI
Primary source: MGI:MGI:1920175
See related: Ensembl:ENSMUSG00000036469 AllianceGenome:MGI:1920175
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: March1; 2900024D24Rik
Summary: Enables ubiquitin protein ligase activity. Involved in antigen processing and presentation of peptide antigen via MHC class II; immune response; and protein polyubiquitination. Located in bounding membrane of organelle; endoplasmic reticulum membrane; and trans-Golgi network membrane. Is expressed in brain; glossopharyngeal ganglion; and spinal cord. Orthologous to human MARCHF1 (membrane associated ring-CH-type finger 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E18 (RPKM 3.6), spleen adult (RPKM 2.6) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 B3.1; 8 33.06 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (66070558..66925761)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (65618009..66472841)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E3 ubiquitin ligase MARCH1 reduces inflammation and pyroptosis in cerebral ischemia-reperfusion injury via PCSK9 downregulation.
Title: E3 ubiquitin ligase MARCH1 reduces inflammation and pyroptosis in cerebral ischemia-reperfusion injury via PCSK9 downregulation.
Targeting psychological stress-steroid-MARCH1 signaling pathway promotes the efficacy of specific allergen immunotherapy.
Title: Targeting psychological stress-steroid-MARCH1 signaling pathway promotes the efficacy of specific allergen immunotherapy.
MARCH1 Controls an Exhaustion-like Program of Effector CD4(+) T Cells Promoting Allergic Airway Inflammation.
Title: MARCH1 Controls an Exhaustion-like Program of Effector CD4(+) T Cells Promoting Allergic Airway Inflammation.
Lymph node-resident dendritic cells drive TH2 cell development involving MARCH1.
Title: Lymph node-resident dendritic cells drive T(H)2 cell development involving MARCH1.
Lack of the E3 Ubiquitin Ligase March1 Affects CD8 T Cell Fate and Exacerbates Insulin Resistance in Obese Mice.
Title: Lack of the E3 Ubiquitin Ligase March1 Affects CD8 T Cell Fate and Exacerbates Insulin Resistance in Obese Mice.
The E3 ubiquitin ligase MARCH1 regulates antimalaria immunity through interferon signaling and T cell activation.
Title: The E3 ubiquitin ligase MARCH1 regulates antimalaria immunity through interferon signaling and T cell activation.
March1 ubiquitin-dependent MHCII turnover is critical to maintain homeostasis of lipid rafts and the tetraspanin web in dendritic cells.
Title: MARCH1 protects the lipid raft and tetraspanin web from MHCII proteotoxicity in dendritic cells.
Results show that MARCH1 deficiency results in sex-specific changes in body weight, adiposity, and increases glucose clearance. Genetic association study for MARCH1 in type 2 diabetes risk in publicly-available datasets found evidence suggestive of association. These data show a link between immune function and diabetes implicating MARCH1 as a regulator of lipid metabolism and glucose tolerance in sex-specific manner.
Title: The E3 ubiquitin ligase MARCH1 regulates glucose-tolerance and lipid storage in a sex-specific manner.
found downstream sequence elements, present in the first coding exon of March-I in antigen-presenting cells (APCs), that confer regulation of March-I expression in activated APCs
Title: A major isoform of the E3 ubiquitin ligase March-I in antigen-presenting cells has regulatory sequences within its gene.
unexpected intersection between MHC I and MHC II such that the surface expression of both molecules are indirectly and directly regulated by MARCH1 ubiquitination, respectively.
Title: MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (1) 1 citation

General gene information

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Markers

D8Mit131 (e-PCR), detects polymorphism
- UniSTS:131334

D8Mit132 (e-PCR), detects polymorphism
- UniSTS:131335

BB314629 (e-PCR)
- UniSTS:207959

RH126212 (e-PCR)
- UniSTS:162920

BB085186 (e-PCR)
- UniSTS:185756

AI256176 (e-PCR)
- UniSTS:208287

D7S2538 (e-PCR)
- UniSTS:20143

D8Dcr27 (e-PCR), detects polymorphism
- UniSTS:507834

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MHC protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables MHC protein binding	ISO Inferred from Sequence Orthology more info
enables MHC protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in antigen processing and presentation of peptide antigen via MHC class II	IBA Inferred from Biological aspect of Ancestor more info
involved_in antigen processing and presentation of peptide antigen via MHC class II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in antigen processing and presentation of peptide antigen via MHC class II	ISO Inferred from Sequence Orthology more info
involved_in immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in immune response	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in early endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in late endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome membrane	ISO Inferred from Sequence Orthology more info
located_in lysosomal membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in lysosome	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in trans-Golgi network membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase MARCHF1

Names: E3 ubiquitin-protein ligase MARCH1; MARCH-I; RING-type E3 ubiquitin transferase MARCH1; RING-type E3 ubiquitin transferase MARCHF1; membrane-associated RING finger protein 1; membrane-associated RING-CH protein I; membrane-associated ring finger (C3HC4) 1

NP_001159844.1

EC 2.3.2.27

NP_001159847.1

EC 2.3.2.27

NP_001280713.1

EC 2.3.2.27

NP_001350052.1

EC 2.3.2.27

NP_001350053.1

EC 2.3.2.27

NP_001350054.1

EC 2.3.2.27

NP_001390802.1

EC 2.3.2.27

NP_001390803.1

EC 2.3.2.27

NP_001390804.1

EC 2.3.2.27

NP_001390805.1

EC 2.3.2.27

NP_001390806.1

EC 2.3.2.27

NP_001390807.1

EC 2.3.2.27

NP_001390808.1

EC 2.3.2.27

NP_001390809.1

EC 2.3.2.27

NP_001390810.1

EC 2.3.2.27

NP_001390811.1

EC 2.3.2.27

NP_001390812.1

EC 2.3.2.27

NP_001390813.1

EC 2.3.2.27

NP_001390814.1

EC 2.3.2.27

NP_001390815.1

EC 2.3.2.27

NP_001390816.1

EC 2.3.2.27

NP_001390818.1

EC 2.3.2.27

NP_001390823.1

EC 2.3.2.27

NP_780397.2

EC 2.3.2.27

XP_006509817.1

EC 2.3.2.27

XP_006509818.1

EC 2.3.2.27

XP_030099653.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001166372.2 → NP_001159844.1 E3 ubiquitin-protein ligase MARCHF1 isoform 1

See identical proteins and their annotated locations for NP_001159844.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction in the 3' coding sequence compared to variant 4. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 4.

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC140272

Consensus CDS

CCDS52560.1

UniProtKB/Swiss-Prot

Q6NZQ8

Related

ENSMUSP00000105884.2, ENSMUST00000110255.8

Conserved Domains (1) summary

cd16698
Location:79 → 130

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001166375.1 → NP_001159847.1 E3 ubiquitin-protein ligase MARCHF1 isoform 2

See identical proteins and their annotated locations for NP_001159847.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction in the 3' coding sequence compared to variant 4. The resulting isoform (2) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 4.

Source sequence(s)

AK137337, AK152592, BB126406, BE686288, BM943350

Consensus CDS

CCDS52561.1

UniProtKB/Swiss-Prot

Q6NZQ8

Conserved Domains (1) summary

smart00744
Location:69 → 117

RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins
NM_001293784.2 → NP_001280713.1 E3 ubiquitin-protein ligase MARCHF1 isoform 4

See identical proteins and their annotated locations for NP_001280713.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) encodes isoform 4.

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC139159, AC140272

Consensus CDS

CCDS80884.1

UniProtKB/Swiss-Prot

Q3U7M8, Q3UVF8, Q6NZQ8, Q8C294, Q8CBA1

Conserved Domains (2) summary

smart00744
Location:79 → 127

RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

pfam15245
Location:40 → 102

VGLL4; Transcription cofactor vestigial-like protein 4
NM_001363123.2 → NP_001350052.1 E3 ubiquitin-protein ligase MARCHF1 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 4.

Source sequence(s)

AC117251, AC122800, AC131655, AC139159, AC140272

Consensus CDS

CCDS22333.1

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001363124.2 → NP_001350053.1 E3 ubiquitin-protein ligase MARCHF1 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (6) uses an alternate in-frame splice junction compared to variant 4.

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC139159, AC140272

Consensus CDS

CCDS52560.1

Conserved Domains (1) summary

cd16698
Location:79 → 130

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001363125.2 → NP_001350054.1 E3 ubiquitin-protein ligase MARCHF1 isoform 5

Status: VALIDATED

Description

Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (5) differs in the N-terminus and lacks an alternate internal segment compared to isoform 4.

Source sequence(s)

AC117251, AC122800, AC131655, AC139159, AC140272

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001403873.1 → NP_001390802.1 E3 ubiquitin-protein ligase MARCHF1 isoform 6

Status: VALIDATED

Source sequence(s)

AC114634, AC122800, AC139159, AC140272

UniProtKB/TrEMBL

D3YVY0

Related

ENSMUSP00000105885.2, ENSMUST00000110256.8
NM_001403874.1 → NP_001390803.1 E3 ubiquitin-protein ligase MARCHF1 isoform 7

Status: VALIDATED

Source sequence(s)

AC117251, AC122800, AC131655, AC139159, AC140272
NM_001403875.1 → NP_001390804.1 E3 ubiquitin-protein ligase MARCHF1 isoform 7

Status: VALIDATED

Source sequence(s)

AC122800, AC139159, AC140272
NM_001403876.1 → NP_001390805.1 E3 ubiquitin-protein ligase MARCHF1 isoform 8

Status: VALIDATED

Source sequence(s)

AC114634, AC122800, AC139159, AC140272
NM_001403877.1 → NP_001390806.1 E3 ubiquitin-protein ligase MARCHF1 isoform 9

Status: VALIDATED

Source sequence(s)

AC122800, AC139159, AC140272
NM_001403878.1 → NP_001390807.1 E3 ubiquitin-protein ligase MARCHF1 isoform 10

Status: VALIDATED

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC139159, AC140272
NM_001403879.1 → NP_001390808.1 E3 ubiquitin-protein ligase MARCHF1 isoform 4

Status: VALIDATED

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC140272

UniProtKB/Swiss-Prot

Q3U7M8, Q3UVF8, Q6NZQ8, Q8C294, Q8CBA1

Related

ENSMUSP00000136545.2, ENSMUST00000178982.8
NM_001403880.1 → NP_001390809.1 E3 ubiquitin-protein ligase MARCHF1 isoform 4

Status: VALIDATED

Source sequence(s)

AC114634, AC117251, AC122800, AC131655, AC139159, AC140272

UniProtKB/Swiss-Prot

Q3U7M8, Q3UVF8, Q6NZQ8, Q8C294, Q8CBA1
NM_001403881.1 → NP_001390810.1 E3 ubiquitin-protein ligase MARCHF1 isoform 4

Status: VALIDATED

Source sequence(s)

AC114634, AC122800, AC139159, AC140272

UniProtKB/Swiss-Prot

Q3U7M8, Q3UVF8, Q6NZQ8, Q8C294, Q8CBA1
NM_001403882.1 → NP_001390811.1 E3 ubiquitin-protein ligase MARCHF1 isoform 4

Status: VALIDATED

Source sequence(s)

AC114634, AC122800, AC139159, AC140272

UniProtKB/Swiss-Prot

Q3U7M8, Q3UVF8, Q6NZQ8, Q8C294, Q8CBA1
NM_001403883.1 → NP_001390812.1 E3 ubiquitin-protein ligase MARCHF1 isoform 3

Status: VALIDATED

Source sequence(s)

AC122800, AC139159, AC140272
NM_001403884.1 → NP_001390813.1 E3 ubiquitin-protein ligase MARCHF1 isoform 3

Status: VALIDATED

Source sequence(s)

AC122800, AC139159, AC140272

Related

ENSMUSP00000072302.7, ENSMUST00000072482.13
NM_001403885.1 → NP_001390814.1 E3 ubiquitin-protein ligase MARCHF1 isoform 1

Status: VALIDATED

Source sequence(s)

AC114634, AC122800, AC139159, AC140272
NM_001403886.1 → NP_001390815.1 E3 ubiquitin-protein ligase MARCHF1 isoform 5

Status: VALIDATED

Source sequence(s)

AC117251, AC122800, AC131655, AC139159, AC140272
NM_001403887.1 → NP_001390816.1 E3 ubiquitin-protein ligase MARCHF1 isoform 5

Status: VALIDATED

Source sequence(s)

AC122800, AC139159, AC140272
NM_001403889.1 → NP_001390818.1 E3 ubiquitin-protein ligase MARCHF1 isoform 11

Status: VALIDATED

Source sequence(s)

AC122800

Related

ENSMUSP00000105882.3, ENSMUST00000110253.3
NM_001403894.1 → NP_001390823.1 E3 ubiquitin-protein ligase MARCHF1 isoform 12

Status: VALIDATED

Source sequence(s)

AC122800

Related

ENSMUSP00000044070.6, ENSMUST00000039540.12
NM_175188.5 → NP_780397.2 E3 ubiquitin-protein ligase MARCHF1 isoform 3

See identical proteins and their annotated locations for NP_780397.2

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate first exon and lacks an alternate internal exon compared to variant 4. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 4.

Source sequence(s)

AC117251, AC122800, AC131655, AC139159, AC140272

Consensus CDS

CCDS22333.1

UniProtKB/Swiss-Prot

Q6NZQ8

Related

ENSMUSP00000105888.2, ENSMUST00000110259.8

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

66070558..66925761

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030243793.2 → XP_030099653.1 E3 ubiquitin-protein ligase MARCHF1 isoform X5

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
XM_006509754.5 → XP_006509817.1 E3 ubiquitin-protein ligase MARCHF1 isoform X1

Conserved Domains (1) summary

cd16698
Location:243 → 294

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
XM_006509755.3 → XP_006509818.1 E3 ubiquitin-protein ligase MARCHF1 isoform X2

Conserved Domains (1) summary

cd16698
Location:243 → 294

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins