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    Asap2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Mus musculus (house mouse) ]

    Gene ID: 211914, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Asap2provided by MGI
    Official Full Name
    ArfGAP with SH3 domain, ankyrin repeat and PH domain 2provided by MGI
    Primary source
    MGI:MGI:2685438
    See related
    Ensembl:ENSMUSG00000052632 AllianceGenome:MGI:2685438
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PAP; PAG3; Ddef2; Gm592; Gm1523; 6530401G17Rik
    Summary
    Predicted to enable GTPase activator activity. Predicted to be involved in positive regulation of GTPase activity. Predicted to be located in cytosol and plasma membrane. Is expressed in brain and liver. Orthologous to human ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in CNS E14 (RPKM 10.8), bladder adult (RPKM 10.1) and 27 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Asap2 in Genome Data Viewer
    Location:
    12 A1.3; 12 8.29 cM
    Exon count:
    31
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (21161369..21320172)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (21111368..21270171)

    Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 3110053B16 gene Neighboring gene STARR-seq mESC enhancer starr_31716 Neighboring gene STARR-seq mESC enhancer starr_31717 Neighboring gene predicted gene 5977 Neighboring gene STARR-seq mESC enhancer starr_31718 Neighboring gene STARR-seq mESC enhancer starr_31719 Neighboring gene STARR-seq mESC enhancer starr_31721 Neighboring gene STARR-seq mESC enhancer starr_31722 Neighboring gene predicted gene, 40855 Neighboring gene integrin beta 1 binding protein 1 Neighboring gene predicted gene, 25821 Neighboring gene cleavage and polyadenylation specificity factor 3 Neighboring gene a disintegrin and metallopeptidase domain 17 Neighboring gene isoamyl acetate-hydrolyzing esterase 1 homolog

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. Mandiyan V, et al. EMBO J, 1999 Dec 15. PMID 10601011, Free PMC Article
    2. PAG3/Papalpha/KIAA0400, a GTPase-activating protein for ADP-ribosylation factor (ARF), regulates ARF6 in Fcgamma receptor-mediated phagocytosis of macrophages. Uchida H, et al. J Exp Med, 2001 Apr 16. PMID 11304556, Free PMC Article
    3. Selenoprotein K regulation of palmitoylation and calpain cleavage of ASAP2 is required for efficient FcγR-mediated phagocytosis. Norton RL, et al. J Leukoc Biol, 2017 Feb. PMID 27601625, Free PMC Article
    4. Quantitative comparison of CrkL-SH3 binding proteins from embryonic murine brain and liver: Implications for developmental signaling and the quantification of protein species variants in bottom-up proteomics. Cheerathodi M, et al. J Proteomics, 2015 Jul 1. PMID 25982384, Free PMC Article
    5. Neuregulin1 (NRG1) signaling through Fyn modulates NMDA receptor phosphorylation: differential synaptic function in NRG1+/- knock-outs compared with wild-type mice. Bjarnadottir M, et al. J Neurosci, 2007 Apr 25. PMID 17460065, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. these findings identify ASAP2 as a new target of Selk-dependent palmitoylation and reveal a new mechanism regulating the efficiency of FcgammaR-mediated phagocytosis.
      Title: Selenoprotein K regulation of palmitoylation and calpain cleavage of ASAP2 is required for efficient FcγR-mediated phagocytosis.
    2. In conclusion, we have identified that ARL15 acts as an insulin-sensitizing effector molecule to upregulate the phosphorylation of members of the canonical IR/IRS1/PDPK1/AKT insulin pathway by interacting with its GAP ASAP2 and activating PDPK1. This research may provide new insights into GTPase-mediated insulin signalling regulation and facilitate the development of new pharmacotherapeutic targets for insulin sensitizati
      Title: ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (4) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC90837

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables GTPase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi cisterna membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cellular_component ND
    No biological Data available
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
    Names
    development and differentiation enhancing factor 2
    paxillin-associated protein with ARF GAP activity 3
    pyk2 C-terminus-associated protein

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001004364.2 → NP_001004364.2  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks two alternate in-frame exons, compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform c.
      Source sequence(s)
      BC080847, BC096022
      Consensus CDS
      CCDS49036.1
      UniProtKB/TrEMBL
      D3YX85, Q3UH05
      Related
      ENSMUSP00000054631.10, ENSMUST00000050990.10
      Conserved Domains (8) summary
      cd11966
      Location:897 → 952
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:579 → 673
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:297 → 403
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:306 → 393
      PH; PH domain
      pfam01412
      Location:421 → 538
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:607 → 661
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:586 → 618
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34 → 248
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

    2. NM_001098168.1 → NP_001091637.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform a

      See identical proteins and their annotated locations for NP_001091637.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform c.
      Source sequence(s)
      BC080847, BC096022
      Consensus CDS
      CCDS56832.1
      UniProtKB/Swiss-Prot
      Q501K1, Q66JN2, Q7SIG6
      UniProtKB/TrEMBL
      Q3UH05
      Related
      ENSMUSP00000099098.5, ENSMUST00000101562.11
      Conserved Domains (8) summary
      cd11966
      Location:900 → 955
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:582 → 676
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:300 → 406
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:309 → 396
      PH; PH domain
      pfam01412
      Location:424 → 541
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:610 → 664
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:589 → 621
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34 → 248
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

    3. NM_001135192.1 → NP_001128664.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform c

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c).
      Source sequence(s)
      AC156032, AK147622, BC096022, CD351639
      Consensus CDS
      CCDS49037.1
      UniProtKB/TrEMBL
      E9PX52, Q3UH27
      Related
      ENSMUSP00000063217.9, ENSMUST00000064595.15
      Conserved Domains (8) summary
      cd07642
      Location:34 → 248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:942 → 997
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:579 → 673
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:297 → 403
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:306 → 393
      PH; PH domain
      pfam01412
      Location:421 → 538
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:607 → 661
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:586 → 618
      ANK; ANK repeat [structural motif]

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000078.7 Reference GRCm39 C57BL/6J

      Range
      21161369..21320172
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006515047.2 → XP_006515110.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1

      UniProtKB/TrEMBL
      Q3UH27
      Conserved Domains (6) summary
      cd07642
      Location:34 → 248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:945 → 1000
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd13251
      Location:300 → 406
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      sd00045
      Location:589 → 621
      ANK; ANK repeat [structural motif]
      cd08849
      Location:422 → 544
      ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
      pfam12796
      Location:592 → 676
      Ank_2; Ankyrin repeats (3 copies)

    2. XM_006515048.2 → XP_006515111.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2

      UniProtKB/TrEMBL
      Q3UH27
      Conserved Domains (6) summary
      cd07642
      Location:34 → 248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:942 → 997
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd13251
      Location:297 → 403
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      sd00045
      Location:586 → 618
      ANK; ANK repeat [structural motif]
      cd08849
      Location:419 → 541
      ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
      pfam12796
      Location:589 → 673
      Ank_2; Ankyrin repeats (3 copies)

    3. XM_036157300.1 → XP_036013193.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4

      Conserved Domains (2) summary
      cl12013
      Location:34 → 248
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17171
      Location:300 → 354
      PH-like; Pleckstrin homology-like domain

    4. XM_036157302.1 → XP_036013195.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X6

      Conserved Domains (1) summary
      cl12013
      Location:34 → 248
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

    5. XM_036157301.1 → XP_036013194.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X5

      Conserved Domains (2) summary
      cl12013
      Location:34 → 248
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17171
      Location:297 → 351
      PH-like; Pleckstrin homology-like domain

    6. XM_030246641.2 → XP_030102501.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3

      UniProtKB/TrEMBL
      Q3UH27
      Conserved Domains (6) summary
      cd11966
      Location:827 → 882
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd13251
      Location:182 → 288
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      sd00045
      Location:471 → 503
      ANK; ANK repeat [structural motif]
      cd08849
      Location:304 → 426
      ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
      pfam12796
      Location:474 → 558
      Ank_2; Ankyrin repeats (3 copies)
      cl12013
      Location:1 → 130
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

    RNA

    1. XR_001780443.2 RNA Sequence

    2. XR_004937480.1 RNA Sequence

    3. XR_004937481.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q7SIG6.3 GenPept UniProtKB/Swiss-Prot:Q7SIG6

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