Nfatc3 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nfatc3provided by MGI
Official Full Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3provided by MGI
Primary source: MGI:MGI:103296
See related: Ensembl:ENSMUSG00000031902 AllianceGenome:MGI:103296
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NFAT4; NFATx; D8Ertd281e
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; chromatin binding activity; and transcription cis-regulatory region binding activity. Involved in T cell differentiation in thymus; positive regulation of artery morphogenesis; and positive regulation of biosynthetic process. Acts upstream of or within several processes, including calcineurin-NFAT signaling cascade; cardiac muscle hypertrophy in response to stress; and circulatory system development. Located in cytosol and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and lung. Orthologous to human NFATC3 (nuclear factor of activated T cells 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in thymus adult (RPKM 43.7), spleen adult (RPKM 12.4) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 D3; 8 53.08 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (106785450..106857169)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (106058818..106130537)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identification of PAX6 and NFAT4 as the Transcriptional Regulators of the Long Noncoding RNA Mrhl in Neuronal Progenitors.
Title: Identification of PAX6 and NFAT4 as the Transcriptional Regulators of the Long Noncoding RNA Mrhl in Neuronal Progenitors.
Macrophage NFATc3 prevents foam cell formation and atherosclerosis: evidence and mechanisms.
Title: Macrophage NFATc3 prevents foam cell formation and atherosclerosis: evidence and mechanisms.
Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition.
Title: Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition.
NFATc3-dependent expression of miR-153-3p promotes mitochondrial fragmentation in cardiac hypertrophy by impairing mitofusin-1 expression.
Title: NFATc3-dependent expression of miR-153-3p promotes mitochondrial fragmentation in cardiac hypertrophy by impairing mitofusin-1 expression.
Orai1 inhibitor STIM2beta regulates myogenesis by controlling SOCE dependent transcriptional factors.
Title: Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.
data indicated that NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.
Title: LncRNA NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.
NFATc3-deletion attenuated metabolism syndrome, reduced inflammatory regulators expression, inactivated NF-kappaB (p65), Caspase-3 and p38/JNK signaling pathway.
Title: NFATc3 deficiency protects against high fat diet (HFD)-induced hypothalamus inflammation and apoptosis via p38 and JNK suppression.
FZD5 is a receptor for SFRP2 and mediates SFRP2-induced angiogenesis via calcineurin/NFATc3 pathway in endothelial cells.
Title: Frizzled-5: a high affinity receptor for secreted frizzled-related protein-2 activation of nuclear factor of activated T-cells c3 signaling to promote angiogenesis.
Redirected splicing of calcineurin A to the fetal isoforms in adult muscle and in differentiated C2C12 slows the timing of muscle relaxation, promotes nuclear localization of calcineurin target Nfatc3, and/or affects expression of Nfatc transcription targets.
Title: Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions.
Our study demonstrates that Orai1-Ca(2+)-calcineurin-NFATc4 signaling is an essential inflammatory pathway required for TNFalpha-induced endothelial cell activation and vascular inflammation. Therefore, Orai1 may be a potential therapeutic target for treatment of inflammatory diseases.
Title: A new comprehensive method for detection of livestock-related pathogenic viruses using a target enrichment system.

Submit: New GeneRIF Correction See all GeneRIFs (68)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C80703 (e-PCR)
- UniSTS:179810

Nfatc3 (e-PCR), detects polymorphism
- UniSTS:144004

D8Ertd281e (e-PCR), detects polymorphism
- UniSTS:167558

Nfatc3 (e-PCR), detects polymorphism
- UniSTS:499101

PMC263836P1 (e-PCR)
- UniSTS:272329

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription factor binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DN4 thymocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within branching involved in blood vessel morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in calcineurin-NFAT signaling cascade	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcineurin-NFAT signaling cascade	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cardiac muscle hypertrophy in response to stress	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular respiration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to calcium ion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to lithium ion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within heart development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within myotube cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myotube differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular associated smooth muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of artery morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive thymic T cell selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of store-operated calcium entry	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within skeletal muscle fiber development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within thymus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within vascular associated smooth muscle cell development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within vascular associated smooth muscle cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: nuclear factor of activated T-cells, cytoplasmic 3

Names: NF-AT4; NF-ATc3; T-cell transcription factor NFAT4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001368796.1 → NP_001355725.1 nuclear factor of activated T-cells, cytoplasmic 3 isoform 2

Status: VALIDATED

Source sequence(s)

AC133195, AC133499, EU887629

UniProtKB/Swiss-Prot

P97305, Q60896

UniProtKB/TrEMBL

B5B2U4

Conserved Domains (3) summary

cd07881
Location:422 → 595

RHD-n_NFAT; N-terminal sub-domain of the Rel homology domain (RHD) of nuclear factor of activated T-cells (NFAT) proteins

cd01178
Location:600 → 700

IPT_NFAT; IPT domain of the NFAT family of transcription factors. NFAT transcription complexes are a target of calcineurin, a calcium dependent phosphatase, and activate genes mainly involved in cell-cell-interaction.

pfam12287
Location:705 → 848

Caprin-1_C; Cytoplasmic activation/proliferation-associated protein-1 C term
NM_001368797.1 → NP_001355726.1 nuclear factor of activated T-cells, cytoplasmic 3 isoform 3

Status: VALIDATED

Source sequence(s)

AC133195, AC133499, EU887626

Consensus CDS

CCDS90454.1

UniProtKB/TrEMBL

B5B2U1, B5B2U2

Related

ENSMUSP00000148556.2, ENSMUST00000211991.2

Conserved Domains (3) summary

cd07881
Location:414 → 587

RHD-n_NFAT; N-terminal sub-domain of the Rel homology domain (RHD) of nuclear factor of activated T-cells (NFAT) proteins

cd01178
Location:592 → 692

IPT_NFAT; IPT domain of the NFAT family of transcription factors. NFAT transcription complexes are a target of calcineurin, a calcium dependent phosphatase, and activate genes mainly involved in cell-cell-interaction.

pfam12287
Location:697 → 840

Caprin-1_C; Cytoplasmic activation/proliferation-associated protein-1 C term
NM_010901.3 → NP_035031.2 nuclear factor of activated T-cells, cytoplasmic 3 isoform 1

See identical proteins and their annotated locations for NP_035031.2

Status: VALIDATED

Source sequence(s)

AC133195, AC133499

Consensus CDS

CCDS22628.1

UniProtKB/Swiss-Prot

P97305, Q60896

UniProtKB/TrEMBL

Q3UZ64

Related

ENSMUSP00000104931.2, ENSMUST00000109308.3

Conserved Domains (2) summary

cd07881
Location:422 → 595

RHD-n_NFAT; N-terminal sub-domain of the Rel homology domain (RHD) of nuclear factor of activated T-cells (NFAT) proteins

cd01178
Location:600 → 700

IPT_NFAT; IPT domain of the NFAT family of transcription factors. NFAT transcription complexes are a target of calcineurin, a calcium dependent phosphatase, and activate genes mainly involved in cell-cell-interaction.

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

106785450..106857169

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036153795.1 → XP_036009688.1 nuclear factor of activated T-cells, cytoplasmic 3 isoform X2

UniProtKB/Swiss-Prot

P97305, Q60896

Conserved Domains (3) summary

cd07881
Location:422 → 595

RHD-n_NFAT; N-terminal sub-domain of the Rel homology domain (RHD) of nuclear factor of activated T-cells (NFAT) proteins

cl15674
Location:594 → 653

IPT; Immunoglobulin-like fold, Plexins, Transcription factors (IPT). IPTs are also known as Transcription factor ImmunoGlobin (TIG) domains. They are present in intracellular transcription factors, cell surface receptors (such as plexins and scatter factor ...

cl41732
Location:238 → 309

KLF6_7_N-like; N-terminal domain of Kruppel-like factor (KLF) 6, KLF7, and similar proteins
XM_006530754.5 → XP_006530817.1 nuclear factor of activated T-cells, cytoplasmic 3 isoform X1

UniProtKB/Swiss-Prot

P97305, Q60896

Conserved Domains (2) summary

cd07881
Location:422 → 595

RHD-n_NFAT; N-terminal sub-domain of the Rel homology domain (RHD) of nuclear factor of activated T-cells (NFAT) proteins

cd01178
Location:600 → 700

IPT_NFAT; IPT domain of the NFAT family of transcription factors. NFAT transcription complexes are a target of calcineurin, a calcium dependent phosphatase, and activate genes mainly involved in cell-cell-interaction.