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    RNF185 ring finger protein 185 [ Homo sapiens (human) ]

    Gene ID: 91445, updated on 27-Aug-2024

    Summary

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    Official Symbol
    RNF185provided by HGNC
    Official Full Name
    ring finger protein 185provided by HGNC
    Primary source
    HGNC:HGNC:26783
    See related
    Ensembl:ENSG00000138942 MIM:620096; AllianceGenome:HGNC:26783
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in positive regulation of ERAD pathway; protein autoubiquitination; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in thyroid (RPKM 37.5), prostate (RPKM 19.7) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    22q12.2
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (31160182..31207019)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (31623670..31670571)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (31556168..31603005)

    Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene Sharpr-MPRA regulatory region 9733 Neighboring gene SNRPN pseudogene 2 Neighboring gene microRNA 3928 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr22:31579617-31580130 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:31580131-31580643 Neighboring gene origin of replication in RNF185 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18849 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13624 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31608967-31609892 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18850 Neighboring gene LIM domain kinase 2 Neighboring gene RNA, U6 small nuclear 1128, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18851 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31643479-31644369 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31644370-31645259 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31672194-31672774 Neighboring gene protein phosphatase 1 regulatory inhibitor subunit 14B pseudogene 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587. Lin K, et al. J Transl Med, 2022 Feb 19. PMID 35183197, Free PMC Article
    2. RNF185 modulates JWA ubiquitination and promotes gastric cancer metastasis. Qiu D, et al. Biochim Biophys Acta Mol Basis Dis, 2018 May. PMID 29481911
    3. The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation. Zhou Y, et al. Biochem Biophys Res Commun, 2014 May 9. PMID 24727453
    4. The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response. Wang Q, et al. PLoS Pathog, 2017 Mar. PMID 28273161, Free PMC Article
    5. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. Tang F, et al. PLoS One, 2011. PMID 21931693, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RNF185 Control of COL3A1 Expression Limits Prostate Cancer Migration and Metastatic Potential.
      Title: RNF185 Control of COL3A1 Expression Limits Prostate Cancer Migration and Metastatic Potential.
    2. CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.
      Title: CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.
    3. increased RNF185 expression facilitated GC cell migration in vitro and promoted GC metastasis in vivo by downregulating JWA expression.
      Title: RNF185 modulates JWA ubiquitination and promotes gastric cancer metastasis.
    4. this study uncovers RNF185 as the first E3 ubiquitin ligase of cyclic GMP-AMP synthase (cGAS), shedding light on the regulation of cGAS activity in innate immune responses
      Title: The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response.
    5. these results indicate that RNF185 negatively regulates osteogenesis through the degradation of Dvl2 and down-regulation of canonical Wnt signaling pathway and suggest a possible therapeutic target in osteoporosis.
      Title: The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation.
    6. data thus identify RNF185 and RNF5 as a novel E3 ligase module that is central to the control of CFTR degradation.
      Title: RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
    7. Human BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo.
      Title: RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat HIV-1 Tat downregulates the expression of ring finger protein 185 (RNF185) in human primary T cells PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ38628

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-containing complex binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity TAS
    Traceable Author Statement
    more info
    		  
    enables ubiquitin-like protein conjugating enzyme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin-like protein conjugating enzyme binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ERAD pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ERAD pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in autophagy IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in defense response to virus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of ERAD pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of innate immune response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of type I interferon-mediated signaling pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein K27-linked ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein autoubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transmembrane transport TAS
    Traceable Author Statement
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in mitochondrial outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase RNF185
    Names
    BSK65-MONO1
    BSK65-MONO2
    BSK65-PANC1
    BSK65-PANC2
    BSK65-TEST1
    BSK65-TEST2
    BSK65-TEST3
    RING-type E3 ubiquitin transferase RNF185
    NP_001129297.1
    NP_689480.2

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001135825.2 → NP_001129297.1  E3 ubiquitin-protein ligase RNF185 isoform 2

      See identical proteins and their annotated locations for NP_001129297.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) is lacking two in-frame coding exons compared to transcript variant 1, resulting in a shorter isoform (2) missing a 56 aa protein segment compared to isoform 1.
      Source sequence(s)
      AA258379, DB134817, DQ296562
      Consensus CDS
      CCDS46689.1
      Related
      ENSP00000266252.7, ENST00000266252.8
      Conserved Domains (1) summary
      cl17238
      Location:37 → 64
      RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain

    2. NM_152267.4 → NP_689480.2  E3 ubiquitin-protein ligase RNF185 isoform 1

      See identical proteins and their annotated locations for NP_689480.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1).
      Source sequence(s)
      AA258379, BC033166, DB134817
      Consensus CDS
      CCDS13890.1
      UniProtKB/Swiss-Prot
      A8K5C1, A9X3T8, Q8N900, Q96GF1
      Related
      ENSP00000320508.5, ENST00000326132.11
      Conserved Domains (1) summary
      cd16744
      Location:38 → 80
      RING-HC_RNF185; RING finger, HC subclass, found in RING finger protein 185 (RNF185) and similar proteins

    RNA

    1. NR_024209.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) is missing an internal coding exon and contains an additional exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
      Source sequence(s)
      AA258379, BC033166, BX648019, DB134817

    2. NR_024210.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) uses an alternate donor splice site at one of the coding exons compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is truncated.
      Source sequence(s)
      AA258379, BC033166, DA498404, DB134817

    3. NR_024211.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) is missing an internal coding exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
      Source sequence(s)
      AA258379, BC033166, BX648019, DB134817

    4. NR_024212.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7) is missing a coding exon (containing the translation start site) compared to transcript variant 1. This results in the use of a downstream AUG in a different frame, and a predicted protein that is severely truncated with no similarity to this gene product. However, this transcript is sufficiently abundant to represent as a RefSeq record.
      Source sequence(s)
      AA258379, BC033166, DB134817
      Related
      ENST00000471384.5

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

      Range
      31160182..31207019
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060946.1 Alternate T2T-CHM13v2.0

      Range
      31623670..31670571
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001135824.1: Suppressed sequence

      Description
      NM_001135824.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript.
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96GF1.1 GenPept UniProtKB/Swiss-Prot:Q96GF1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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