APN2 DNA-(apurinic or apyrimidinic site) lyase APN2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: APN2
Official Full Name: DNA-(apurinic or apyrimidinic site) lyase APN2
Primary source: SGD:S000000115
Locus tag: YBL019W
See related: AllianceGenome:SGD:S000000115; FungiDB:YBL019W; VEuPathDB:YBL019W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ETH1
Summary: Enables DNA-(apurinic or apyrimidinic site) endonuclease activity; double-stranded DNA 3'-5' DNA exonuclease activity; and phosphoric diester hydrolase activity. Involved in base-excision repair. Predicted to be active in nucleus. Orthologous to human APEX2 (apurinic/apyrimidinic endodeoxyribonuclease 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See APN2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (184353..185915)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Kinetic Features of 3'-5'-Exonuclease Activity of Apurinic/Apyrimidinic Endonuclease Apn2 from Saccharomyces cerevisiae.
Title: Kinetic Features of 3'-5'-Exonuclease Activity of Apurinic/Apyrimidinic Endonuclease Apn2 from Saccharomyces cerevisiae.
The APE2 Exonuclease Is a Client of the Hsp70-Hsp90 Axis in Yeast and Mammalian Cells.
Title: The APE2 Exonuclease Is a Client of the Hsp70-Hsp90 Axis in Yeast and Mammalian Cells.
The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source.
Title: The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source.
Genetic and biochemical analyses reveal that Apn2 resolves phosphotyrosine-DNA conjugates, terminal 2',3'-cyclic phosphates, and their hydrolyzed products. APN2 also suppresses 2-base pair slippage mutagenesis in RNH201-deficient cells.
Title: Apn2 resolves blocked 3' ends and suppresses Top1-induced mutagenesis at genomic rNMP sites.
Direct physical evidence that Apn2 not only repair cellular base damage but also prevent break accumulation that can result from abasic sites being channeled into other base excision repair pathway(s).
Title: Apn1 and Apn2 endonucleases prevent accumulation of repair-associated DNA breaks in budding yeast as revealed by direct chromosomal analysis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA 3'-5' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoric diester hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in base-excision repair	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-(apurinic or apyrimidinic site) lyase APN2

NP_009534.1

EC 3.1.11.2

Class II abasic (AP) endonuclease involved in repair of DNA damage; also possesses 3'-diesterase and PCNA-stiimulated 3' to 5' exonuclease activities; resolves blocked 3' ends that result from removal of misincorporated nucleotides; frays and cleaves 3' DNA termini facilitating 3' DNA-end healing via endonucleolytic cleavage; homolog of human APEX2 and E. coli exoIII

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

184353..185915

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178259.1 → NP_009534.1 TPA: DNA-(apurinic or apyrimidinic site) lyase APN2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009534.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPY1, E9P926, P38207

UniProtKB/TrEMBL

A6ZKS9, G2W8Z0, N1PAY0

Conserved Domains (2) summary

cd09088
Location:18 → 361

Ape2-like_AP-endo; Human Ape2-like subfamily of the ExoIII family purinic/apyrimidinic (AP) endonucleases

pfam06839
Location:474 → 520

zf-GRF; GRF zinc finger