SNF7 ESCRT-III subunit protein SNF7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SNF7
Official Full Name: ESCRT-III subunit protein SNF7
Primary source: SGD:S000004015
Locus tag: YLR025W
See related: AllianceGenome:SGD:S000004015; FungiDB:YLR025W; VEuPathDB:YLR025W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DID1; RNS4; VPL5; VPS32
Summary: Involved in several processes, including ATP export; ESCRT III complex assembly; and intralumenal vesicle formation. Located in cytoplasm and plasma membrane. Part of ESCRT III complex. Human ortholog(s) of this gene implicated in cataract 31 multiple types. Orthologous to several human genes including CHMP4A (charged multivesicular body protein 4A); CHMP4B (charged multivesicular body protein 4B); and CHMP4C (charged multivesicular body protein 4C). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SNF7 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (194452..195174)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Three-dimensional architecture of ESCRT-III flat spirals on the membrane.
Title: Three-dimensional architecture of ESCRT-III flat spirals on the membrane.
The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors in S. cerevisiae.
Title: The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors in S. cerevisiae.
The major ESCRT-III subunit Snf7 localizes transiently to vesicles and tubules surrounded by a cup-shaped membrane and this was accelerated in vps4Delta cells, correlating with increased Acb1 secretion.
Title: ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion.
Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly.
Title: ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis.
Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block endosomal sorting complexes required for transport function.
Title: Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.
Snf7 alpha6 helix is crucial for binding of Bro1 and Rim20 in vivo and is indispensable for the multivesicular body sorting.
Title: Interaction maps of the Saccharomyces cerevisiae ESCRT-III protein Snf7.
Binding of Vps4 to ESCRT-III, coordinated by interactions with Vps2 and Snf7, is coupled to membrane neck constriction during intraluminal vesicle formation.
Title: Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation.
we demonstrate that the N terminus of Snf7 has been tuned to maintain the topological constraints associated with ESCRT-III-filament-mediated membrane invagination and vesicle formation.
Title: Essential N-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation.
By generating SNF7 mutations that severely affect endocytic trafficking, but leave the Rim pathway function intact, data show that the two functions of SNF7 can be separated genetically.
Title: Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression.
Snf7 influences SUC2 gene expression through activation of the Rim101 pathway.
Title: ESCRT-III protein Snf7 mediates high-level expression of the SUC2 gene via the Rim101 pathway.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in ATP export	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ESCRT III complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in intralumenal vesicle formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in reticulophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in vacuolar transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle budding from membrane	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of ESCRT III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ESCRT III complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in multivesicular body	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ESCRT-III subunit protein SNF7

NP_013125.1

One of four core subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; subunit of the endosomal sorting complex required for transport III (ESCRT-III) that forms flat spirals on lipid monolayers as part of the complexes role in driving membrane abscission