TOP2 DNA topoisomerase 2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TOP2
Gene description: DNA topoisomerase 2
Primary source: FungiDB:YNL088W
Locus tag: YNL088W
See related: SGD:S000005032; AllianceGenome:SGD:S000005032; VEuPathDB:YNL088W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TOR3; TRF3
Summary: Enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity. Involved in chromatin organization; nucleic acid metabolic process; and regulation of DNA metabolic process. Located in DNA replication termination region and synaptonemal complex. Used to study acute myeloid leukemia and cancer. Orthologous to human TOP2A (DNA topoisomerase II alpha) and TOP2B (DNA topoisomerase II beta). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TOP2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (457704..461990)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Assessing acute myeloid leukemia susceptibility in rearrangement-driven patients by DNA breakage at topoisomerase II and CCCTC-binding factor/cohesin binding sites.
Title: Assessing acute myeloid leukemia susceptibility in rearrangement-driven patients by DNA breakage at topoisomerase II and CCCTC-binding factor/cohesin binding sites.
Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes.
Title: Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes.
Topoisomerases Modulate the Timing of Meiotic DNA Breakage and Chromosome Morphogenesis in Saccharomyces cerevisiae.
Title: Topoisomerases Modulate the Timing of Meiotic DNA Breakage and Chromosome Morphogenesis in Saccharomyces cerevisiae.
Trapped topoisomerase II initiates formation of de novo duplications via the nonhomologous end-joining pathway in yeast.
Title: Trapped topoisomerase II initiates formation of de novo duplications via the nonhomologous end-joining pathway in yeast.
Data highlights the role of Uls1 in antagonizing Top2 function. Remodelling Top2 binding is thus an important new means by which Snf2 enzymes promote genome stability.
Title: The ATP-dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning.
Reaction rates for individual steps in the DNA cleavage reaction are characterized. Kinetic pathway of divalent ions in the DNA cleavage reaction is revealed. Sequence-preferential DNA bending is induced for the cleavage site selection by TOP2.
Title: Selection of DNA Cleavage Sites by Topoisomerase II Results from Enzyme-Induced Flexibility of DNA.
We show that KDM4B modulates TOP2 accessibility to chromatin, elucidating a mechanism of TOP2 inhibitor sensitivity. These findings indicate that CRGs mediate anthracycline benefit by altering DNA accessibility, with implications for the stratification of patients with breast cancer and treatment decision making
Title: Chromatin regulators mediate anthracycline sensitivity in breast cancer.
Condensin DNA supercoiling activity correlates with Top2 intertwine removal during DNA replication.
Title: Physical Proximity of Sister Chromatids Promotes Top2-Dependent Intertwining.
Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers.
Title: Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination.
Topoisomerase II plays a central role in a molecular pathway for crossover interference
Title: Topoisomerase II mediates meiotic crossover interference.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
involved_in DNA topological change	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling at centromere	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiosis I cell cycle process	IEA Inferred from Electronic Annotation more info
involved_in rRNA transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork progression beyond termination site	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in DNA replication termination region	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in synaptonemal complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase 2

NP_014311.3

EC 5.6.2.2

Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

457704..461990

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182926.3 → NP_014311.3 TPA: DNA topoisomerase 2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014311.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W191, P06786

UniProtKB/TrEMBL

A6ZRZ2, E9P8H4, E9P8H8, N1NXH6, Q7LMA0

Conserved Domains (1) summary

PTZ00108
Location:10 → 1428

PTZ00108; DNA topoisomerase 2-like protein; Provisional