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    PRP18 mRNA splicing protein PRP18 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852889, updated on 18-Sep-2024

    Summary

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    Official Symbol
    PRP18
    Official Full Name
    mRNA splicing protein PRP18
    Primary source
    SGD:S000003238
    Locus tag
    YGR006W
    See related
    AllianceGenome:SGD:S000003238; FungiDB:YGR006W; VEuPathDB:YGR006W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Contributes to second spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for second transesterification step and nuclear retention of unspliced pre-mRNA at the site of transcription. Part of U2-type post-spliceosomal complex; U4/U6 x U5 tri-snRNP complex; and U5 snRNP. Orthologous to human PRPF18 (pre-mRNA processing factor 18). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See PRP18 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (506070..506825)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene peroxisome biogenesis protein Neighboring gene transcription factor IIF subunit TFG2 Neighboring gene ethanolamine-phosphate cytidylyltransferase Neighboring gene ATPase-stabilizing factor family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing. Crotti LB, et al. Genes Dev, 2007 May 15. PMID 17504938, Free PMC Article
    2. Crystal structure of the functional domain of the splicing factor Prp18. Jiang J, et al. Proc Natl Acad Sci U S A, 2000 Mar 28. PMID 10737784, Free PMC Article
    3. A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Horowitz DS, et al. Mol Cell Biol, 1993 May. PMID 8474454, Free PMC Article
    4. PRP18, a protein required for the second reaction in pre-mRNA splicing. Vijayraghavan U, et al. Mol Cell Biol, 1990 Jan. PMID 2403639, Free PMC Article
    5. Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18. Bacíková D, et al. RNA, 2002 Oct. PMID 12403466, Free PMC Article

    See all (69) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
      Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.
    2. structure along with sequence comparisons among SRA1p orthologs and against authentic RNA recognition motif (RRM) proteins indicates that it is not an RRM domain but rather an all-helical protein with a fold more similar to the PRP18 splicing factor
      Title: The C-terminal domain of SRA1p has a fold more similar to PRP18 than to an RRM and does not directly bind to the SRA1 RNA STR7 region.
    3. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.
      Title: The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.
    4. Arginine1753 of Prp8 collaborates with Prp18 to stabilize U5 snRNA/exon contacts that are important for the second transesterification step of pre-mRNA splicing.
      Title: Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.
    5. Prp18 acts in concert with U5 snRNA during the second step of pre-mRNA splicing.
      Title: Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    contributes_to second spliceosomal transesterification activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to second spliceosomal transesterification activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for second transesterification step IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in generation of catalytic spliceosome for second transesterification step IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nuclear mRNA surveillance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in nuclear mRNA surveillance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of U2-type post-spliceosomal complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U2-type post-spliceosomal complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of U4/U6 x U5 tri-snRNP complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U4/U6 x U5 tri-snRNP complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of U5 snRNP IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U5 snRNP IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mRNA splicing protein PRP18
    NP_011520.2
    • Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      506070..506825
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181135.1 → NP_011520.2  TPA: mRNA splicing protein PRP18 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011520.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VUE3, P33411, Q02459, Q05724
      UniProtKB/TrEMBL
      A6ZUZ8, B3LIL0, C7GK76, C8Z8Q0, G2WE99, N1P217
      Conserved Domains (1) summary
      pfam02840
      Location:116 → 243
      Prp18; Prp18 domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P33411.3

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