MSN5 karyopherin MSN5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSN5
Official Full Name: karyopherin MSN5
Primary source: SGD:S000002743
Locus tag: YDR335W
See related: AllianceGenome:SGD:S000002743; FungiDB:YDR335W; VEuPathDB:YDR335W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KAP142; STE21
Summary: Enables nuclear export signal receptor activity. Involved in protein export from nucleus and tRNA re-export from nucleus. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in breast cancer; gastrointestinal system cancer (multiple); lung carcinoma (multiple); multiple myeloma; and primary ovarian insufficiency. Orthologous to human XPO5 (exportin 5). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See MSN5 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1141168..1144842)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Msn5 inactivation causes a shift of CLN2 and SWI5 mRNAs from heavy-polysomal to light-polysomal and non-polysomal fractions, supporting a defect in Cln2 and Swi5 protein synthesis in the msn5 mutant. Msn5 is required for the proper protein synthesis of specific proteins, introducing a new level of control of cell cycle regulators.
Title: Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.
defining regions of Ste5 that mediate nuclear export; Msn5 (homolog of human Exportin-5) and one or more exportins or adaptor molecules besides Crm1/Xpo1 may regulate Ste5 through multiple recognition sites
Title: Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.
Subcellular distributions of Msn5 and Los1 are altered upon glucose deprivation but not amino acid deprivation.
Title: Separate responses of karyopherins to glucose and amino acid availability regulate nucleocytoplasmic transport.
These data provide evidence that the export of Msn5 is likely mediated by a specific subset of FG-Nups and that the GLFG repeat domain of Nup100 is important for Msn5-mediated nuclear protein export.
Title: A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.
Data show that Utp9p is an intranuclear component of the Msn5p-mediated nuclear tRNA export pathway, and suggest that Utp9p and Utp8p translocate aminoacyl-tRNAs from the nucleolus to Msn5p and assist with formation of the Msn5p-tRNA-Gsp1p-GTP complex.
Title: Utp9p facilitates Msn5p-mediated nuclear reexport of retrograded tRNAs in Saccharomyces cerevisiae.
nuclear export of Whi5 is assisted by beta-karyopherin Msn5, and there is physical interaction between Whi5 and Msn5
Title: Spatial regulation of the start repressor Whi5.
Msn5-mediated nuclear export of Ho
Title: Nuclear export of Ho endonuclease of yeast via Msn5.
phosphorylation of Maf1 inside the nucleus acts both directly by decreasing of Maf1-mediated repression of Pol III and indirectly by stimulation of Msn5 binding and export of nuclear Maf1 to the cytoplasm
Title: Derepression of RNA polymerase III transcription by phosphorylation and nuclear export of its negative regulator, Maf1.
Nup82 is important for Msn5-mediated nuclear protein export.
Title: The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and performs a function distinct from translocation in RPA protein import.
The carrier Msn5p/Kap142p promotes nuclear export of the hsp70 Ssa4p and relocates in response to stress.
Title: The carrier Msn5p/Kap142p promotes nuclear export of the hsp70 Ssa4p and relocates in response to stress.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear export signal receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear export signal receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables nuclear export signal receptor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA export from nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein export from nucleus	IDA Inferred from Direct Assay more info	PubMed
involved_in protein export from nucleus	IEA Inferred from Electronic Annotation more info
involved_in protein export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA re-export from nucleus	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of RNA nuclear export complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: karyopherin MSN5

NP_010622.1

Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1141168..1144842

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180643.1 → NP_010622.1 TPA: karyopherin MSN5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010622.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSW7, P52918

UniProtKB/TrEMBL

B3LFW2, B5VGL8, C7GWN2, N1PAI1

Conserved Domains (1) summary

COG5101
Location:23 → 172

CRM1; Importin beta-related nuclear transport receptor [Nuclear structure / Intracellular trafficking and secretion]