SWI4 SBF complex DNA-binding subunit SWI4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SWI4
Official Full Name: SBF complex DNA-binding subunit SWI4
Primary source: SGD:S000000913
Locus tag: YER111C
See related: AllianceGenome:SGD:S000000913; FungiDB:YER111C; VEuPathDB:YER111C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ART1
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific. Involved in positive regulation of transcription from RNA polymerase II promoter in response to heat stress and positive regulation of transcription involved in G1/S transition of mitotic cell cycle. Located in chromatin. Part of SBF transcription complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SWI4 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (382595..385876, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of sphingolipid synthesis by the G1/S transcription factor Swi4.
Title: Regulation of sphingolipid synthesis by the G1/S transcription factor Swi4.
TORC1 regulates G1/S transition and cell proliferation via the E2F homologs MBF and SBF in yeast.
Title: TORC1 regulates G1/S transition and cell proliferation via the E2F homologs MBF and SBF in yeast.
methionine influx triggers Art1 translocation to the plasma membrane (PM), followed by Ppz-mediated dephosphorylation which promotes cargo recognition at the PM.
Title: Methionine triggers Ppz-mediated dephosphorylation of Art1 to promote cargo-specific endocytosis.
Authors show that a functionally similar ART sorting signal is also required for the endocytosis of a second Art1-dependent cargo, Can1, suggesting a common mechanism for recognition of Art1 substrates.
Title: Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex.
expression of mutated Swi4(S159A) protein in the GG104-Swi4Delta strain did not restore the transient G1 arrest induced by PKA activation, suggesting that Swi4 phosphorylation by PKA regulates CLN1 gene expression and G1/S phase transition.
Title: The transcription factor Swi4 is target for PKA regulation of cell size at the G1 to S transition in Saccharomyces cerevisiae.
Swi4/Swi6 (SBF) factor binding at HO follows a temporal cascade, with SBF bound at upstream regulatory sequence (URS2-L) serving to relay a signal from URS1 to the SBF sites in URS2-R that ultimately activate gene expression.
Title: Spatiotemporal cascade of transcription factor binding required for promoter activation.
We found that the transcriptional cofactor Swi6p and activator Swi4p, as well as the G(2)/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment
Title: Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae.
Study shows that Npr1 inhibits the activity of Art1, an arrestin-like adaptor protein that promotes endocytosis by targeting the Rsp5 ubiquitin ligase to specific plasma membrane cargoes.
Title: TORC1 regulates endocytosis via Npr1-mediated phosphoinhibition of a ubiquitin ligase adaptor.
The dimeric complex of Mpk1 binds to a Swi4 recognition site in the FKS2 promoter.[Mpk1, Swi4, FKS2]
Title: Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.
Data show that phosphorylation of Ser423 specifically blocked SBF activation by preventing Mpk1 association with Swi4, revealing a novel mechanism for regulating MAPK target specificity.
Title: Mechanism of Mpk1 mitogen-activated protein kinase binding to the Swi4 transcription factor and its regulation by a novel caffeine-induced phosphorylation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables sequence-specific DNA binding	HDA more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MBF transcription complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of SBF transcription complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SBF transcription complex	IDA Inferred from Direct Assay more info	PubMed
part_of SBF transcription complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of SBF transcription complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SBF complex DNA-binding subunit SWI4

NP_011036.1

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

382595..385876 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179001.1 → NP_011036.1 TPA: SBF complex DNA-binding subunit SWI4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011036.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM17, P25302

UniProtKB/TrEMBL

B5VHN6, C7GS75

Conserved Domains (4) summary

PHA03095
Location:512 → 684

PHA03095; ankyrin-like protein; Provisional

COG1196
Location:813 → 1091

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

sd00045
Location:521 → 551

ANK; ANK repeat [structural motif]

pfam04383
Location:52 → 123

KilA-N; KilA-N domain