VTA1 Vta1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VTA1
Official Full Name: Vta1p
Primary source: SGD:S000004171
Locus tag: YLR181C
See related: AllianceGenome:SGD:S000004171; FungiDB:YLR181C; VEuPathDB:YLR181C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ATPase activator activity and protein-macromolecule adaptor activity. Involved in late endosome to vacuole transport via multivesicular body sorting pathway. Located in multivesicular body. Colocalizes with endosome membrane. Orthologous to human VTA1 (vesicle trafficking 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See VTA1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (516678..517670, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the Did2 binding does not result in Vta1NTD significant conformational changes, revealing that Did2, similar to Vps60, enhances Vta1 stimulation of Vps4 ATPase activity in an indirect manner.
Title: NMR studies on the interactions between yeast Vta1 and Did2 during the multivesicular bodies sorting pathway.
Four Vps4 subunits form a helix whose interfaces are consistent with ATP binding, is stabilized by Vta1, and binds the substrate peptide.
Title: Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase.
ESCRT-III binding to the Vta1 microtubule interacting and trafficking domains relieves linker region autoinhibition of the VSE to produce maximal activation of Vps4 during ESCRT function.
Title: Relief of autoinhibition enhances Vta1 activation of Vps4 via the Vps4 stimulatory element.
Structural basis of molecular recognition between ESCRT-III-like protein Vps60 and AAA-ATPase regulator Vta1 in the multivesicular body pathway.
Title: Structural basis of molecular recognition between ESCRT-III-like protein Vps60 and AAA-ATPase regulator Vta1 in the multivesicular body pathway.
impact of Vta1 and ESCRT-III family members on Vps4 ATPase activity
Title: ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1.
mechanism of regulation by Vta1 in which the C-terminal domain stabilizes the ATP-dependent double ring assembly.
Title: Structural basis of Vta1 function in the multivesicular body sorting pathway.
models in which Vps4p MIT and Vta1p domains engage endosomal sorting complex required for transport-III substrates above the bowl and help transfer them into the bowl to be pumped through the center of the dodecameric assembly
Title: Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p.
Vps46p regulates the membrane association of Vps4p and is required for the interaction of Vta1p with Vps32p/Snf7p of the ESCRT-III complex.
Title: Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body.
Regulates disassembly of endosomal sorting complex required for transport (ESCRT) proteins through Vps4.
Title: Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables ATPase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IEA Inferred from Electronic Annotation more info
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endosome	HDA more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in multivesicular body	IBA Inferred from Biological aspect of Ancestor more info
located_in multivesicular body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Vta1p

NP_013282.1

Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

516678..517670 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182068.1 → NP_013282.1 TPA: Vta1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013282.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYI5, Q06263, Q07057

UniProtKB/TrEMBL

A7A172, C7GIX0, N1P6R5

Conserved Domains (2) summary

pfam04652
Location:10 → 161

Vta1; Vta1 like

pfam18097
Location:294 → 328

Vta1_C; Vta1 C-terminal domain