TEF1 translation elongation factor EF-1 alpha [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TEF1
Gene description: translation elongation factor EF-1 alpha
Primary source: FungiDB:YPR080W
Locus tag: YPR080W
See related: SGD:S000006284; AllianceGenome:SGD:S000006284; VEuPathDB:YPR080W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables several functions, including guanyl ribonucleotide binding activity; melatonin binding activity; and small GTPase binding activity. Involved in actin filament bundle assembly; tRNA export from nucleus; and translational elongation. Located in fungal-type vacuole membrane and mitochondrion. Human ortholog(s) of this gene implicated in autosomal dominant non-syndromic intellectual disability 38; developmental and epileptic encephalopathy 33; and ovarian cancer. Orthologous to several human genes including EEF1A2 (eukaryotic translation elongation factor 1 alpha 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TEF1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (700594..701970)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Domain II of the translation elongation factor eEF1A is required for Gcn2 kinase inhibition.
Title: Domain II of the translation elongation factor eEF1A is required for Gcn2 kinase inhibition.
Loss of EF1A methylation was not lethal but resulted in reduced growth rates, particularly under caffeine and rapamycin stress conditions.
Title: Protein Methylation and Translation: Role of Lysine Modification on the Function of Yeast Elongation Factor 1A.
Data indicate that the methylation of lysine (Lys) in elongation factor 1A (eEF1A) by methyltransferase is conserved from yeast to human.
Title: Novel N-terminal and Lysine Methyltransferases That Target Translation Elongation Factor 1A in Yeast and Human.
Ynl024c is the enzyme responsible for methylation of eEF1A at Lys390.
Title: Saccharomyces cerevisiae Eukaryotic Elongation Factor 1A (eEF1A) Is Methylated at Lys-390 by a METTL21-Like Methyltransferase.
Tef1/2 (the yeast form of translation elongation factor 1alpha [eEF1A]) aids the specificity of Msn5 for aminoacylated tRNAs to form a quaternary complex consisting of Msn5, RanGTP, aminoacylated tRNA, and Tef1/2
Title: In vivo biochemical analyses reveal distinct roles of β-importins and eEF1A in tRNA subcellular traffic.
the Rho1p-eEF1A complex acts to spatially localize a pool of eEF1A to vacuoles where it can readily organize F-actin.
Title: Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodeling.
protein lysine methyltransferase Efm5 (Ygr001c) is required for trimethylation of Lys-79 on EF1A
Title: A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A.
Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2alpha phosphorylation.
Title: Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2α phosphorylation.
Methylation of TEF1 by See1 facilitates tombusvirus replication in Saccharomyces cerevisiae.
Title: Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants.
Elongation factor 1A (EF-1A) competes with the RTD pathway for substrate tRNAs, since its overexpression suppresses the tRNA degradation and the growth defect of strains subject to RTD, whereas reduced levels of EF-1A have the opposite effect.
Title: The yeast rapid tRNA decay pathway competes with elongation factor 1A for substrate tRNAs and acts on tRNAs lacking one or more of several modifications.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GDP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables melatonin binding	IDA Inferred from Direct Assay more info	PubMed
enables translation elongation factor activity	IEA Inferred from Electronic Annotation more info
enables translation elongation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament bundle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in tRNA export from nucleus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in tRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: translation elongation factor EF-1 alpha

NP_015405.1

Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus