rec12 DNA topoisomerase type II (DSB-forming) Rec12/Spo11 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: rec12
Official Full Name: DNA topoisomerase type II (DSB-forming) Rec12/Spo11
Primary source: PomBase:SPAC17A5.11
Locus tag: SPOM_SPAC17A5.11
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: spo11
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Genomic context

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See rec12 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 5

Sequence:: Chromosome: I; NC_003424.3 (1772925..1774203)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8.
Title: Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8.
Reductions in rec10 rec12 double deletion crosses indicate partially redundant functions of Rec10 and Rec12 in the initiation of intrachromosomal recombination.
Title: Rec10- and Rec12-independent recombination in meiosis of Schizosaccharomyces pombe.
Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32(Mre11)) nuclease activity is required for the removal of Rec12(Spo11).
Title: Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions.
Rec12 binding was also detected in centromeric core regions, which raises the intriguing possibility that Rec12 plays additional roles in meiotic chromosome dynamics.
Title: Sites of strong Rec12/Spo11 binding in the fission yeast genome are associated with meiotic recombination and with centromeres.
Fission yeast the genome-wide distribution of meiosis-specific Rec12-DNA linkages.
Title: A discrete class of intergenic DNA dictates meiotic DNA break hotspots in fission yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	ISM Inferred from Sequence Model more info
enables ATP hydrolysis activity	IC Inferred by Curator more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA end binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in homologous chromosome orientation in meiotic metaphase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	HDA more info	PubMed
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase type II (DSB-forming) Rec12/Spo11

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

1772925..1774203

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001018912.1 → NP_593479.1 DNA topoisomerase type II (DSB-forming) Rec12/Spo11 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_593479.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P40384

Conserved Domains (3) summary

cd00223
Location:173 → 328

TOPRIM_TopoIIB_SPO; TOPRIM_TopoIIB_SPO: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in the type IIB family of DNA topoisomerases and Spo11. This subgroup contains proteins similar to Sulfolobus shibatae topoisomerase VI (TopoVI) ...

COG1697
Location:1 → 343

Spo11; DNA topoisomerase VI, subunit A [Replication, recombination and repair]

pfam04406
Location:69 → 130

TP6A_N; Type IIB DNA topoisomerase