Cma1 chymase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cma1provided by RGD
Official Full Name: chymase 1provided by RGD
Primary source: RGD:2365
See related: EnsemblRapid:ENSRNOG00000020563 AllianceGenome:RGD:2365
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: MCP3P; Mcpt5
Summary: Enables peptide binding activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to glucose stimulus; midbrain development; and positive regulation of angiogenesis. Located in extracellular region. Biomarker of renovascular hypertension. Human ortholog(s) of this gene implicated in asthma; atopic dermatitis; hypertrophic cardiomyopathy; and sarcoidosis. Orthologous to human CMA1 (chymase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Muscle (RPKM 66.2), Thymus (RPKM 37.9) and 7 other tissues See more
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Genomic context

Location:: 15p12

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (33387351..33390133, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (29417451..29420233, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (34601037..34603819, complement)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chymase activity in synovial fluids of collagen-induced-arthritis rats regulates gelatinase release and promotes synovial fibroblasts proliferation via FAK/p21 signaling pathway.
Title: Mast cell chymase in synovial fluid of collagen-induced-arthritis rats regulates gelatinase release and promotes synovial fibroblasts proliferation via FAK/p21 signaling pathway.
The pathobiologic response in WKY rats was directly linked to increases in cardiac gene expression and enzymatic activity of chymase and modest reductions in ACE2 activity.
Title: Blunting of cardioprotective actions of estrogen in female rodent heart linked to altered expression of cardiac tissue chymase and ACE2.
Compared to chymase, ACE-mediated angiotensin-(1-12) metabolism in the heart was several fold lower. Overall our findings suggest that angiotensin-(1-12), not Ang I, is the better substrate for Ang II formation by chymase in adult rats.
Title: Primacy of cardiac chymase over angiotensin converting enzyme as an angiotensin-(1-12) metabolizing enzyme.
In a pure VO model, chymase produced in adult cardiac fibroblasts leads to autophagic degradation of newly synthesized intracellular procollagen I, suggesting a new role of chymase in extracellular matrix degradation.
Title: Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload.
The results indicate an important role for chymase in inducing fibrosis through TGF-beta1 activation, parallel with Ang II effects.
Title: Role of chymase in diabetic nephropathy.
These results suggest that chymase can promote cardiac fibroblast proliferation and collagen synthesis via TGF-beta 1/Smad pathway rather than angiotensin II, which is implicated in the process of myocardial fibrosis.
Title: Chymase induces profibrotic response via transforming growth factor-beta 1/Smad activation in rat cardiac fibroblasts.
chymase inactivates the FAK-mediated cell survival signaling
Title: Activated mast cells induce endothelial cell apoptosis by a combined action of chymase and tumor necrosis factor-alpha.
Based on the results, this study suggested rMCP-5 is a key promoter of angiogenesis in the rat.
Title: Inhibition of granuloma-associated angiogenesis by controlling mast cell mediator release: role of mast cell protease-5.
Plasma ANG II does not appear to regulate intrarenal chymase activity. ACE-independent ANG II production by chymase may provide mechanism for augmenting intrarenal ANG II in ischemic kidney in renovascular hypertension.
Title: Angiotensin converting enzyme-independent angiotensin ii production by chymase is up-regulated in the ischemic kidney in renovascular hypertension.
Chymase-secreting mast cells mediate apoptosis of neighboring smooth muscle cells through a mechanism involving degradation of pericellular fibronectin and disruption of the p-focal adhesion kinase-dependent cell-survival signaling cascade.
Title: Mast cell chymase induces smooth muscle cell apoptosis by a mechanism involving fibronectin degradation and disruption of focal adhesions.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Markers

RH94861 (e-PCR)
- UniSTS:120107

Cma1 (e-PCR), detects polymorphism
- UniSTS:536689

RH131228 (e-PCR)
- UniSTS:214511

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables peptidase activity	NAS Non-traceable Author Statement more info	PubMed
enables peptide binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables serine-type peptidase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in basement membrane disassembly	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glucose stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cytokine precursor processing	ISO Inferred from Sequence Orthology more info
involved_in extracellular matrix disassembly	ISO Inferred from Sequence Orthology more info
involved_in midbrain development	IEP Inferred from Expression Pattern more info	PubMed
involved_in peptide metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in positive regulation of angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein maturation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein processing	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of inflammatory response	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasmic ribonucleoprotein granule	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: chymase

Names: alpha-chymase; chymase 1, mast cell; mast cell protease 3; mast cell protease 5; mast cell protease III; rMCP-3; rMCP-5; rMCP-III

NP_037224.1

EC 3.4.21.39

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013092.2 → NP_037224.1 chymase precursor

See identical proteins and their annotated locations for NP_037224.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000015

UniProtKB/Swiss-Prot

P50339, Q9R2C8

UniProtKB/TrEMBL

A6KH67

Related

ENSRNOP00000061330.2, ENSRNOT00000067539.4

Conserved Domains (2) summary

smart00020
Location:21 → 240

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:22 → 243

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...