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    Top3a DNA topoisomerase III alpha [ Rattus norvegicus (Norway rat) ]

    Gene ID: 303194, updated on 10-Jul-2024

    Summary

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    Official Symbol
    Top3aprovided by RGD
    Official Full Name
    DNA topoisomerase III alphaprovided by RGD
    Primary source
    RGD:1309320
    See related
    EnsemblRapid:ENSRNOG00000005099 AllianceGenome:RGD:1309320
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Predicted to enable DNA topoisomerase type I (single strand cut, ATP-independent) activity and single-stranded DNA binding activity. Predicted to be involved in DNA topological change; chromosome separation; and mitochondrial DNA metabolic process. Predicted to be located in PML body. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Adrenal (RPKM 110.0), Testes (RPKM 67.0) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Top3a in Genome Data Viewer
    Location:
    10q22
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 10 NC_086028.1 (45915625..45956856, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 10 NC_051345.1 (45419219..45457356, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 10 NC_005109.4 (46980646..47018728, complement)

    Chromosome 10 - NC_086028.1Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA SNORA15 Neighboring gene FLII, actin remodeling protein Neighboring gene mitochondrial elongation factor 2 Neighboring gene SMCR8-C9orf72 complex subunit Neighboring gene serine hydroxymethyltransferase 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Mutations in TOP3A Cause a Bloom Syndrome-like Disorder. Martin CA, et al. Am J Hum Genet, 2018 Aug 2. PMID 30057030, Free PMC Article
    2. Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA. Nicholls TJ, et al. Mol Cell, 2018 Jan 4. PMID 29290614, Free PMC Article
    3. Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. Yang J, et al. J Biol Chem, 2010 Jul 9. PMID 20445207, Free PMC Article
    4. Association of the Bloom syndrome protein with topoisomerase IIIalpha in somatic and meiotic cells. Johnson FB, et al. Cancer Res, 2000 Mar 1. PMID 10728666
    5. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, et al. Brief Bioinform, 2011 Sep. PMID 21873635, Free PMC Article

    GeneRIFs: Gene References Into Functions

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables single-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in chromosome separation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in double-strand break repair via homologous recombination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in mitochondrial DNA metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in resolution of DNA recombination intermediates ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in PML body ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of RecQ family helicase-topoisomerase III complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of RecQ family helicase-topoisomerase III complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    DNA topoisomerase 3-alpha
    Names
    topoisomerase (DNA) III alpha
    NP_001414385.1
    XP_038943192.1
    XP_038943193.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001427456.2 → NP_001414385.1  DNA topoisomerase 3-alpha

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000010
      UniProtKB/TrEMBL
      D4A6J4
      Related
      ENSRNOP00000061212.5, ENSRNOT00000068351.5

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086028.1 Reference GRCr8

      Range
      45915625..45956856 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039087265.2 → XP_038943193.1  DNA topoisomerase 3-alpha isoform X2

      Conserved Domains (3) summary
      cd03362
      Location:35 → 193
      TOPRIM_TopoIA_TopoIII; The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA ...
      cd00186
      Location:202 → 617
      TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
      pfam01396
      Location:655 → 673
      zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger

    2. XM_039087264.2 → XP_038943192.1  DNA topoisomerase 3-alpha isoform X1

      UniProtKB/TrEMBL
      D4A6J4
      Conserved Domains (4) summary
      cd03362
      Location:8 → 138
      TOPRIM_TopoIA_TopoIII; The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA ...
      cd00186
      Location:147 → 562
      TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
      pfam01396
      Location:600 → 639
      zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger
      pfam06839
      Location:843 → 886
      zf-GRF; GRF zinc finger
    Nucleotide Protein Strain
    Heading Accession and Version

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