PHO8 alkaline phosphatase PHO8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PHO8
Gene description: alkaline phosphatase PHO8
Primary source: FungiDB:YDR481C
Locus tag: YDR481C
See related: SGD:S000002889; AllianceGenome:SGD:S000002889; VEuPathDB:YDR481C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables alkaline phosphatase activity. Involved in nicotinamide nucleotide metabolic process and protein dephosphorylation. Located in fungal-type vacuole membrane. Used to study several diseases, including bone disease (multiple); cryptorchidism; germ cell cancer (multiple); hypophosphatasia (multiple); and primary biliary cholangitis. Human ortholog(s) of this gene implicated in adult hypophosphatasia; childhood hypophosphatasia; hypophosphatasia; infantile hypophosphatasia; and inflammatory bowel disease. Orthologous to several human genes including ALPL (alkaline phosphatase, biomineralization associated) and ALPP (alkaline phosphatase, placental). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PHO8 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1418550..1420250, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
Title: Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
Results suggest that alkaline phosphatase ALP, which is encoded by PHO8, affects the polyphosphate (polyp) content, but not the chain length, and participates in polyP homeostasis in phosphate -sufficient conditions
Title: PHO8 gene coding alkaline phosphatase of Saccharomyces cerevisiae is involved in polyphosphate metabolism.
Pho8 activity and accumulation are zinc-dependent and decrease in zinc-limited cells.
Title: Zinc status and vacuolar zinc transporters control alkaline phosphatase accumulation and activity in Saccharomyces cerevisiae.
PHO8 promoter has greater nucleosome positioning power and the properly positioned nucleosomes are more stable than those at the PHO5 promoter.
Title: Nucleosome stability at the yeast PHO5 and PHO8 promoters correlates with differential cofactor requirements for chromatin opening.
Results show that the histone chaperone Asf1 increases the rate of histone eviction at the PHO5 and PHO8 promoters.
Title: The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters.
A truncated form of alkaline phosphatase Pho8 lacking 62 amino acids from the N-terminus was found to be able to convert farnesyl diphosphate (FPP) into E,E-farnesol.
Title: A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E-farnesol.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables alkaline phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alkaline phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables alkaline phosphatase activity	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 6-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in dephosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in nicotinamide nucleotide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nucleotide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in organophosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphate-containing compound metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alkaline phosphatase PHO8

NP_010769.3

EC 3.1.3.1

EC 3.1.3.54

EC 3.1.7.6

Repressible vacuolar alkaline phosphatase; controls polyphosphate content; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1418550..1420250 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180789.3 → NP_010769.3 TPA: alkaline phosphatase PHO8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010769.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTA4, E9P949, P11491, Q03374

UniProtKB/TrEMBL

A0A8H8UP12, B3LFI5, C7GKS0, C8Z639, N1P5R2

Conserved Domains (1) summary

COG1785
Location:8 → 530

PhoA; Alkaline phosphatase [Inorganic ion transport and metabolism, General function prediction only]