ISW1 chromatin-remodeling ATPase ISW1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ISW1
Official Full Name: chromatin-remodeling ATPase ISW1
Primary source: SGD:S000000449
Locus tag: YBR245C
See related: AllianceGenome:SGD:S000000449; FungiDB:YBR245C; VEuPathDB:YBR245C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SGN2
Summary: Enables mRNA 3'-UTR binding activity; rDNA binding activity; and transcription cis-regulatory region binding activity. Contributes to ATP hydrolysis activity; DNA binding activity; and nucleosome binding activity. Involved in several processes, including DNA-templated transcription; negative regulation of IRE1-mediated unfolded protein response; and regulation of transcription by RNA polymerase II. Located in nucleolar chromatin. Part of Isw1a complex and Isw1b complex. Is active in nucleus. Orthologous to human SMARCA1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1) and SMARCA5 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See ISW1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (708150..711539, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.
Title: ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.
Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin.
Title: Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin.
single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells.
Title: [The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae].
Here the authors mapped the H4-tail binding pocket of ISWI. Surprisingly the binding site was adjacent to but not overlapping with the docking site of an auto-regulatory motif, AutoN, in the N-terminal region (NTR) of ISWI, indicating that AutoN does not act as a simple pseudosubstrate as suggested previously.
Title: Concerted regulation of ISWI by an autoinhibitory domain and the H4 N-terminal tail.
Study identifies the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity.
Title: The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis.
The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism.
Title: The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism.
nucleosome mobilizing activity of Isw1a, Isw1b and Isw2 complexes could be significantly influenced by neighboring nucleosomes
Title: Yeast Isw1a and Isw1b exhibit similar nucleosome mobilization capacities for mononucleosomes, but differently mobilize dinucleosome templates.
The nuclease digestion data suggest that Isw1a can also promote ATP-dependent distortion of nucleosome structure, although less efficiently than the Isw1b complex.
Title: Comparison of the Isw1a, Isw1b, and Isw2 nucleosome disrupting activities.
nuclear localization signal is located at the end of the ISW1 C-terminus.
Title: Nuclear import of chromatin remodeler Isw1 is mediated by atypical bipartite cNLS and classical import pathway.
We find that remodelers( Isw1, Isw2, and Chd1 ) occupancy at NDRs and gene bodies is associated with nucleosome turnover and transcriptional elongation rate
Title: ISWI and CHD chromatin remodelers bind promoters but act in gene bodies.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
contributes_to ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
contributes_to DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleosome array spacer activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to nucleosome binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleosome binding	IEA Inferred from Electronic Annotation more info
enables rDNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of IRE1-mediated unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of RNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcriptional start site selection at RNA polymerase II promoter	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in termination of RNA polymerase I transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in termination of RNA polymerase II transcription	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Isw1a complex	IDA Inferred from Direct Assay more info	PubMed
part_of Isw1b complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolar chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: chromatin-remodeling ATPase ISW1

NP_009804.1

ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; binds to 3'UTR of HAC1 mRNA and limits its nuclear export attenuating the UPR

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

708150..711539 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178593.1 → NP_009804.1 TPA: chromatin-remodeling ATPase ISW1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009804.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQP1, P38144

UniProtKB/TrEMBL

A6ZLI4, B3LMR7

Conserved Domains (1) summary

PLN03142
Location:108 → 1057

PLN03142; Probable chromatin-remodeling complex ATPase chain; Provisional