AMOT angiomotin [Homo sapiens (human)] - Gene

Summary

Official Symbol: AMOTprovided by HGNC
Official Full Name: angiomotinprovided by HGNC
Primary source: HGNC:HGNC:17810
See related: Ensembl:ENSG00000126016 MIM:300410; AllianceGenome:HGNC:17810
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Expression: Broad expression in placenta (RPKM 13.4), brain (RPKM 9.3) and 21 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: Xq23

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	X	NC_000023.11 (112774877..112840831, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	X	NC_060947.1 (111226753..111292678, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	X	NC_000023.10 (112018105..112084059, complement)

Chromosome X - NC_000023.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release.
Title: Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release.
Emerging roles for angiomotin in the nervous system.
Title: Emerging roles for angiomotin in the nervous system.
CLIC1 knockout inhibits invasion and migration of gastric cancer by upregulating AMOT-p130 expression.
Title: CLIC1 knockout inhibits invasion and migration of gastric cancer by upregulating AMOT-p130 expression.
Angiomotin regulates budding and spread of Ebola virus.
Title: Angiomotin regulates budding and spread of Ebola virus.
AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells.
Title: AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells.
AMOT is primarily expressed in migratory extravillous trophoblast cells (EVTs) and its expression increases after 10 weeks of gestation when oxygen tension rises and EVT migration/invasion peaks. In preeclampsia, however, AMOT expression is decreased and its localization to migratory fetomaternal interface EVTs is disrupted. JMJD6 an oxygen sensor, positively regulates AMOT via oxygen-dependent lysyl hydroxylation.
Title: Faulty oxygen sensing disrupts angiomotin function in trophoblast cell migration and predisposes to preeclampsia.
Findings that angiomotin (AMOT)-dependent regulation of Yes-associated protein (YAP) directing human pluripotent stem cells (hPSCs) fate suggest the molecular mechanisms by which the microenvironment can induce neural differentiation.
Title: Angiomotin Regulates YAP Localization during Neural Differentiation of Human Pluripotent Stem Cells.
Amot-p130 functions as a tumor suppressor gene in breast cancer, disrupting beta-catenin stability by competing with Axin for binding to tankyrase.
Title: Angiomotin-p130 inhibits β-catenin stability by competing with Axin for binding to tankyrase in breast cancer.
We have been suggested that AmotP130 suppressed the invasion ability through remodelling of the cytoskeleton of breast cancer cells, involving regulation of the Rho pathway. The cytoskeleton-related pathway components may provide novel, clinically therapeutic targets for breast cancer treatment.
Title: AmotP130 regulates Rho GTPase and decreases breast cancer cell mobility.
AMOT, AMOTL1, and AMOTL2 enhance Hippo signaling by stimulating LATS1/2 autophosphorylation and by connecting LATS1/2 with both its activator SAV1-MST1/2 and its substrate YAP.
Title: Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling.

Submit: New GeneRIF Correction See all GeneRIFs (48)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of angiomotin (AMOT) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells	PubMed
Knockdown of Angiomotin (AMOT) by siRNA inhibits HIV-1 release and infectivity, but expression of AMOT-like protein 1 and AMOT-like protein 2 can rescue HIV-1 Gag release	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Pr55(Gag)	gag	Angiomotin (AMOT) depletion inhibits HIV-1 Gag release from cells, but expression of AMOT-like protein 1 and AMOT-like protein 2 can rescue HIV-1 Gag release	PubMed
	gag	Angiomotin (AMOT) stimulates NEDD4L-dependent release of HIV-1 Gag delta PTAP/YP in a does-dependent manner from 293T, HeLa, and Jurkat cells	PubMed
	gag	Angiomotin (AMOT), AMOT-like protein 1, and AMOT-like protein 2 bind directly and specifically to NEDD4L and HIV-1 Gag-delta p6 in protein pull-down experiments	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AFMa275zc9 (e-PCR)
- UniSTS:25101

AMOT (e-PCR)
- UniSTS:498201

WI-13613 (e-PCR)
- UniSTS:81473

G16015 (e-PCR)
- UniSTS:47261

AL035157 (e-PCR)
- UniSTS:94638

G31118 (e-PCR)
- UniSTS:76476

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables angiostatin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in angiogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in blood vessel endothelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in cell migration involved in gastrulation	IEA Inferred from Electronic Annotation more info
involved_in cell-cell junction assembly	TAS Traceable Author Statement more info	PubMed
involved_in chemotaxis	IEA Inferred from Electronic Annotation more info
involved_in establishment of cell polarity involved in ameboidal cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of epithelial cell polarity	IEA Inferred from Electronic Annotation more info
involved_in gastrulation with mouth forming second	IEA Inferred from Electronic Annotation more info
involved_in hippo signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in in utero embryonic development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of angiogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of vascular permeability	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of blood vessel endothelial cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell size	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of embryonic development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of stress fiber assembly	TAS Traceable Author Statement more info	PubMed
involved_in protein localization	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell migration	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of modification of postsynaptic actin cytoskeleton	IEA Inferred from Electronic Annotation more info
involved_in regulation of small GTPase mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in vasculogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of COP9 signalosome	IDA Inferred from Direct Assay more info	PubMed
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
is_active_in bicellular tight junction	IBA Inferred from Biological aspect of Ancestor more info
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell junction	IDA Inferred from Direct Assay more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in endocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: angiomotin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_012628.1 RefSeqGene

Range

4985..70939

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001113490.2 → NP_001106962.1 angiomotin isoform 1

See identical proteins and their annotated locations for NP_001106962.1

Status: REVIEWED

Source sequence(s)

AC004827

Consensus CDS

CCDS48154.1

UniProtKB/Swiss-Prot

Q4VCS5, Q504X5, Q9HD27, Q9UPT1

Related

ENSP00000361027.3, ENST00000371959.9

Conserved Domains (3) summary

COG1196
Location:431 → 676

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK10263
Location:191 → 328

PRK10263; DNA translocase FtsK; Provisional

pfam12240
Location:599 → 806

Angiomotin_C; Angiomotin C terminal
NM_001386998.1 → NP_001373927.1 angiomotin isoform 1

Status: REVIEWED

Source sequence(s)

AC004827

Consensus CDS

CCDS48154.1

UniProtKB/Swiss-Prot

Q4VCS5, Q504X5, Q9HD27, Q9UPT1

Conserved Domains (3) summary

COG1196
Location:431 → 676

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK10263
Location:191 → 328

PRK10263; DNA translocase FtsK; Provisional

pfam12240
Location:599 → 806

Angiomotin_C; Angiomotin C terminal
NM_001386999.1 → NP_001373928.1 angiomotin isoform 1

Status: REVIEWED

Source sequence(s)

AC004827

Consensus CDS

CCDS48154.1

UniProtKB/Swiss-Prot

Q4VCS5, Q504X5, Q9HD27, Q9UPT1

Conserved Domains (3) summary

COG1196
Location:431 → 676

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK10263
Location:191 → 328

PRK10263; DNA translocase FtsK; Provisional

pfam12240
Location:599 → 806

Angiomotin_C; Angiomotin C terminal
NM_133265.5 → NP_573572.1 angiomotin isoform 2

See identical proteins and their annotated locations for NP_573572.1

Status: REVIEWED

Source sequence(s)

AC004827

Consensus CDS

CCDS14563.1

UniProtKB/TrEMBL

A2BDD9

Related

ENSP00000305557.1, ENST00000304758.5

Conserved Domains (1) summary

pfam12240
Location:190 → 393

Angiomotin_C; Angiomotin C terminal