ATH1 alpha,alpha-trehalase ATH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ATH1
Gene description: alpha,alpha-trehalase ATH1
Primary source: FungiDB:YPR026W
Locus tag: YPR026W
See related: SGD:S000006230; AllianceGenome:SGD:S000006230; VEuPathDB:YPR026W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables alpha,alpha-trehalase activity. Involved in trehalose catabolic process and trehalose transport. Located in cell wall-bounded periplasmic space; fungal-type cell wall; and fungal-type vacuole. Orthologous to human PGGHG (protein-glucosylgalactosylhydroxylysine glucosidase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ATH1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (615379..619014)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Saccharomyces cerevisiae alphaalpha-trehalase (ATH1) activity in soluble cell-free extracts increases after 40 degrees C adaptation but decreases when cells return to 28 degrees C. During 40 degrees C, Ath1 is confined into vesicles, avoiding the cleavage of the outside trehalose.
Title: The trehalose protective mechanism during thermal stress in Saccharomyces cerevisiae: the roles of Ath1 and Agt1.
The behaviour of trehalase mutant strains demonstrates the participation of trehalases Nth1p, Nth2p and Ath1p in the mobilization of intracellular trehalose during growth recovery after saline stress.
Title: The three trehalases Nth1p, Nth2p and Ath1p participate in the mobilization of intracellular trehalose required for recovery from saline stress in Saccharomyces cerevisiae.
hybrid Ath1 truncates fused at their C-terminus with invertase revealed that a 131 amino acid N-terminal fragment of Ath1 was sufficient to target the fusion protein to the cell surface, enabling growth of the suc2Delta mutant on sucrose
Title: The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function.
Gene disruption of NTH1 and ATH1 increased tolerance to freezing, heat, high-sugar and ethanol concentration.
Title: [Construction and stress tolerance of trehalase mutant in Saccharomyces cerevisiae].
The authors investigated the regulation of ATH1 transcription and found a clear involvement of the protein kinase Hog1p in the induction of this gene under severe stress conditions, such as high salt.
Title: Acid trehalase is involved in intracellular trehalose mobilization during postdiauxic growth and severe saline stress in Saccharomyces cerevisiae.
We showed that a tps1 mutant defective in the TPS catalytic subunit cultivated on trehalose, or on a dual source of carbon made of galactose and trehalose, accumulated high levels of intracellular trehalose by its Agt1p-mediated transport.
Title: New insights into trehalose metabolism by Saccharomyces cerevisiae: NTH2 encodes a functional cytosolic trehalase, and deletion of TPS1 reveals Ath1p-dependent trehalose mobilization.
Mutagenesis analyses aimed at identifying the unknown targeting signal revealed that the transmembrane domain of Ath1 contains the information sufficient for its selective sequestration into multivesicular body internal vesicles.
Title: The transmembrane domain of acid trehalase mediates ubiquitin-independent multivesicular body pathway sorting.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables alpha,alpha-trehalase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alpha,alpha-trehalase activity	IEA Inferred from Electronic Annotation more info
enables alpha,alpha-trehalase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on glycosyl bonds	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in trehalose catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in trehalose catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in trehalose catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in trehalose transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell wall-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
is_active_in fungal-type cell wall	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type cell wall	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in periplasmic space	IEA Inferred from Electronic Annotation more info
located_in vacuolar lumen	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alpha,alpha-trehalase ATH1

NP_015351.1

EC 3.2.1.28

Acid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

615379..619014

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184123.1 → NP_015351.1 TPA: alpha,alpha-trehalase ATH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015351.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W436, N1P212, P48016

UniProtKB/TrEMBL

A0A8H4F892, A6ZWR9, B5VTI4

Conserved Domains (1) summary

COG1554
Location:111 → 996

ATH1; Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism]