DFG10 putative polyprenol reductase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DFG10
Official Full Name: putative polyprenol reductase
Primary source: SGD:S000001311
Locus tag: YIL049W
See related: AllianceGenome:SGD:S000001311; FungiDB:YIL049W; VEuPathDB:YIL049W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Predicted to enable 3-oxo-5-alpha-steroid 4-dehydrogenase activity and polyprenol reductase activity. Involved in dolichol biosynthetic process; dolichol-linked oligosaccharide biosynthetic process; and pseudohyphal growth. Located in endoplasmic reticulum. Used to study congenital disorder of glycosylation type I. Human ortholog(s) of this gene implicated in Kahrizi syndrome and congenital disorder of glycosylation Iq. Orthologous to human SRD5A3 (steroid 5 alpha-reductase 3). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See DFG10 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (260158..260919)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DFG10 and SRD5A3 Are necessary for conversion of polyprenol to dolichol in yeast, mouse, and human and likely to be the long-sought polyprenol reductase.
Title: SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-oxo-5-alpha-steroid 4-dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables 3-oxo-5-alpha-steroid 4-dehydrogenase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables polyprenol reductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables polyprenol reductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in dolichol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: putative polyprenol reductase

NP_012215.1

EC 1.3.1.94

Probable polyprenol reductase; catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human homolog SRD5A3 confer CDG (Congenital Disorders of Glycosylation); human SRD5A3 can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

260158..260919

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179399.1 → NP_012215.1 TPA: putative polyprenol reductase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012215.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVN2, P40526

UniProtKB/TrEMBL

A6ZVM0, G2WG96

Conserved Domains (1) summary

cl21511
Location:60 → 252

PEMT; Phospholipid methyltransferase