GAL80 transcription regulator GAL80 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GAL80
Official Full Name: transcription regulator GAL80
Primary source: SGD:S000004515
Locus tag: YML051W
See related: AllianceGenome:SGD:S000004515; FungiDB:YML051W; VEuPathDB:YML051W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and kinase inhibitor activity. Involved in galactose metabolic process; negative regulation of kinase activity; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleus. Orthologous to human GFOD1 (glucose-fructose oxidoreductase domain containing 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See GAL80 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (171594..172901)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.
Title: Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.
A double role of the Gal80 N terminus in activation of transcription by Gal4p.
Title: A double role of the Gal80 N terminus in activation of transcription by Gal4p.
Here, the authors demonstrate that altering the epistatic interaction between Gal80p and Gal4p also abolishes the bistability of the GAL/MEL genetic switch, and the switch response to galactose becomes graded instead of binary.
Title: The binary response of the GAL/MEL genetic switch of Saccharomyces cerevisiae is critically dependent on Gal80p-Gal4p interaction.
GAL switch becomes independent of GAL3 by altering the interaction between Gal4p and Gal80p.
Title: Perturbation of the interaction between Gal4p and Gal80p of the Saccharomyces cerevisiae GAL switch results in altered responses to galactose and glucose.
Discuss the effect of Gal3 on the quaternary structure of Gal80 in light of the evidence pointing to multimeric Gal80 as the form required to inhibit Gal4.
Title: Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.
Overproduction of Gal3p or Gal4p mutants with altered Gal80p-binding characteristics restores GAL gene expression in mutants deficient in ATP or NADP(H) synthesis.
Title: Multiple metabolic signals influence GAL gene activation by modulating the interaction of Gal80p with the transcriptional activator Gal4p.
These results suggest that the Dsg1-mediated efficient transcription process of GAL genes may depend on the interaction status between Gal4p and Gal80p.
Title: Alterations in the interaction between GAL4 and GAL80 effect regulation of the yeast GAL regulon mediated by the F box protein Dsg1.
Gal3p moves from the cytoplasm to the nucleus and interact with Gal80p in response to galactose induction.
Title: Localization and interaction of the proteins constituting the GAL genetic switch in Saccharomyces cerevisiae.
A reverse two-hybrid selection for GAL3 mutations that impair the Gal3-Gal80 interaction yielded 12 single-amino-acid substitutions at residues that are predicted to be surface exposed on Gal3.
Title: Genetic evidence for sites of interaction between the Gal3 and Gal80 proteins of the Saccharomyces cerevisiae GAL gene switch.
to understand mechanism of GAL regulatory system, structure of Gal80p with activation domain of Gal4p was determined; structure reveals existence of a dinucleotide mediating interaction between the two; findings suggest NADP plays a key role
Title: NADP regulates the yeast GAL induction system.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in galactose metabolic process	IEA Inferred from Electronic Annotation more info
involved_in galactose metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription regulator GAL80

NP_013661.1

Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

171594..172901

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182409.1 → NP_013661.1 TPA: transcription regulator GAL80 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013661.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZC4, P04387

UniProtKB/TrEMBL

A6ZM16, B3LLK6, B5VP79, C7GS67, C8ZEF7, G2WK06, N1NXY6

Conserved Domains (1) summary

COG0673
Location:17 → 429

MviM; Predicted dehydrogenase [General function prediction only]