IST2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: IST2
Primary source: FungiDB:YBR086C
Locus tag: YBR086C
See related: SGD:S000000290; AllianceGenome:SGD:S000000290; VEuPathDB:YBR086C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables lipid binding activity. Involved in endoplasmic reticulum membrane organization; protein localization to plasma membrane; and regulation of phosphatidylinositol dephosphorylation. Located in cellular bud membrane and cortical endoplasmic reticulum. Human ortholog(s) of this gene implicated in Miyoshi muscular dystrophy 3; autosomal recessive limb-girdle muscular dystrophy type 2L; autosomal recessive spinocerebellar ataxia 10; gnathodiaphyseal dysplasia; and isolated elevated serum creatine phosphokinase levels. Orthologous to several human genes including ANO10 (anoctamin 10) and ANO8 (anoctamin 8). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (420201..423041, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.
Title: Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.
Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration.
Title: Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration.
Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.
Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.
The authors show that, in BY4741 background, the lack of Ist2 results in the accumulation of higher amounts of sodium when the cells are exposed to the presence of this cation, demonstrating the importance of Ist2 for the maintenance of low intracellular levels of toxic sodium.
Title: Lack of cortical endoplasmic reticulum protein Ist2 alters sodium accumulation in Saccharomyces cerevisiae cells.
IST2 localization depends on the presence of a PM-binding domain, an intrinsically disordered linker region of sufficient length.
Title: Intrinsically disordered linker and plasma membrane-binding motif sort Ist2 and Ssy1 to junctions.
Ist2 in the yeast cortical endoplasmic reticulum promotes trafficking of the amino acid transporter Bap2 to the plasma membrane.
Title: Ist2 in the yeast cortical endoplasmic reticulum promotes trafficking of the amino acid transporter Bap2 to the plasma membrane.
Data show that binding of phosphatidylinositol phosphates leads to efficient accumulation of Ist2 at domains of the cortical ER from where the protein may reach the PM independently of the function of the sec-pathway.
Title: Binding of plasma membrane lipids recruits the yeast integral membrane protein Ist2 to the cortical ER.
Results suggest that a direct interaction of the Ist2 sorting signal with lipids at the plasma membrane places Ist2 at contact sites between cortical ER and plasma membrane.
Title: A signal comprising a basic cluster and an amphipathic alpha-helix interacts with lipids and is required for the transport of Ist2 to the yeast cortical ER.
Data suggest that Btn2p, along with Btn1 and Ist2, may play a regulatory role across the cell in response to alterations in the intracellular environment.
Title: Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae.
Based on these observations, we suggest a local synthesis of Ist2p at cortical ER sites, from where the protein is sorted by a novel mechanism to the plasma membrane.
Title: SEC18/NSF-independent, protein-sorting pathway from the yeast cortical ER to the plasma membrane.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables lipid binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chloride transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum membrane organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of phosphatidylinositol dephosphorylation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in cellular bud membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in cortical endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in cortical endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Ist2p

NP_009643.2

Cortical ER protein involved in ER-plasma membrane (PM) tethering; one of 6 proteins (Ist2, Scs2, Scs22, Tcb1, Tcb2, Tcb3) that connect ER to PM, regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1 phosphatase to PI4P; required to locate Osh6, Osh7 in ER/PM contact site to allow phosphatidylserine transport from ER to PM; localizes to mother in small-budded cells, to bud in medium-, large-budded cells; mRNA transported to bud tip by actomyosin-driven process

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

420201..423041 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178434.1 → NP_009643.2 TPA: Ist2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009643.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ87, P38250, P89499

UniProtKB/TrEMBL

A6ZL34, N1P8D4

Conserved Domains (2) summary

PRK13335
Location:779 → 880

PRK13335; superantigen-like protein SSL3; Reviewed

pfam04547
Location:122 → 588

Anoctamin; Calcium-activated chloride channel