MCK1 serine/threonine/tyrosine protein kinase MCK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MCK1
Official Full Name: serine/threonine/tyrosine protein kinase MCK1
Primary source: SGD:S000005251
Locus tag: YNL307C
See related: AllianceGenome:SGD:S000005251; FungiDB:YNL307C; VEuPathDB:YNL307C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CMS1; YPK1
Summary: Enables cyclin binding activity; enzyme inhibitor activity; and protein kinase activity. Involved in several processes, including ascospore formation; double-strand break repair via nonhomologous end joining; and mitotic sister chromatid segregation. Acts upstream of or within mitotic spindle orientation checkpoint signaling. Predicted to be active in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See MCK1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (56446..57573, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint.
Title: The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint.
Study identified that a conserved GSK-3 kinase Mck1 cooperates with Dun1 in regulating replication threats response in yeasts and reveal the division-of-labor between Mck1 and Dun1 at the S-phase checkpoint pathway to fine-tune dNTP homeostasis.
Title: Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats.
Mck1 identified as a novel positive regulator of inositol de novo synthesis in yeast. Mck1 is required for normal activity of myo-inositol phosphate synthase.
Title: MCK1 is a novel regulator of myo-inositol phosphate synthase (MIPS) that is required for inhibition of inositol synthesis by the mood stabilizer valproate.
ur data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast.
Title: Chronological Lifespan in Yeast Is Dependent on the Accumulation of Storage Carbohydrates Mediated by Yak1, Mck1 and Rim15 Kinases.
Mck1 promotes cell separation and, together with Rim15, controls the cell size after the diauxic shift.
Title: The Yeast GSK-3 Homologue Mck1 Is a Key Controller of Quiescence Entry and Chronological Lifespan.
Mck1 kinase ensures proper DNA replication, prevents DNA damage, and maintains genome integrity by inhibiting Cdc6.
Title: Cdc6 degradation requires phosphodegron created by GSK-3 and Cdk1 for SCFCdc4 recognition in Saccharomyces cerevisiae.
a screen of single-deletion mutants lacking each of the four GSK3 isoforms found that MCK1 is responsible for most of the Elo2 phosphorylation
Title: TORC1 inhibits GSK3-mediated Elo2 phosphorylation to regulate very long chain fatty acid synthesis and autophagy.
We propose that both CDK and Mck1 kinases are required for Cdc6 degradation to ensure a tight control of DNA replication.
Title: A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication.
Phosphorylation of purified Clb2 by Cdk1 is inhibited by catalytically active Mck1 but not catalytically inactive Mck1 in vitro.
Title: The Mck1 GSK-3 kinase inhibits the activity of Clb2-Cdk1 post-nuclear division.
Data show a mechanism through which the conserved Pkh-Ypk kinase cascade and Cdc55-PP2A facilitate rapid, transient sphingolipid production in response to heat stress through Orm protein phosphoregulation.
Title: Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cyclin binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine/tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine/tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine/tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ascospore formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic spindle orientation checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cellular_component	ND No biological Data available more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: serine/threonine/tyrosine protein kinase MCK1

NP_014092.1

EC 2.7.12.1

Dual-specificity S/T and tyrosine protein kinase; spindle position checkpoint component that works in parallel with Kin4p to prevent mitotic exit network (MEN) activation by the Cdc14 early anaphase release (FEAR) network; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent degradation of Rcn1p and Cdc6p, inhibition of protein kinase A, transcriptional regulation, inhibition of RNAPIII, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

56446..57573 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183145.1 → NP_014092.1 TPA: serine/threonine/tyrosine protein kinase MCK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014092.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0N8, P21965

UniProtKB/TrEMBL

A6ZSH3, B3LPE1, B5VQB3, C7GWQ4, C8ZGL7, G2WLG2, N1NZH1

Conserved Domains (1) summary

cd14137
Location:39 → 330

STKc_GSK3; The catalytic domain of the Serine/Threonine Kinase, Glycogen Synthase Kinase 3