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    MMS21 SUMO ligase MMS21 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856695, updated on 28-Oct-2024

    Summary

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    Official Symbol
    MMS21
    Official Full Name
    SUMO ligase MMS21
    Primary source
    SGD:S000000745
    Locus tag
    YEL019C
    See related
    AllianceGenome:SGD:S000000745; FungiDB:YEL019C; VEuPathDB:YEL019C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    NSE2; PSO10
    Summary
    Enables SUMO transferase activity and protein serine/threonine kinase inhibitor activity. Involved in DNA double-strand break attachment to nuclear envelope and DNA repair. Located in nuclear envelope. Part of Smc5-Smc6 complex. Human ortholog(s) of this gene implicated in Seckel syndrome 10. Orthologous to human NSMCE2 (NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase). [provided by Alliance of Genome Resources, Oct 2024]
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    Genomic context

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    See MMS21 in Genome Data Viewer
    Location:
    chromosome: V
    Exon count:
    1
    Sequence:
    Chromosome: V; NC_001137.3 (120498..121301, complement)

    Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene putative indolepyruvate decarboxylase family protein Neighboring gene Eaf5p Neighboring gene proteolipid ATPase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001137.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Increased spontaneous mitotic segregation in MMS-sensitive mutants of Saccharomyces cerevisiae. Prakash S, et al. Genetics, 1977 Oct. PMID 200524, Free PMC Article
    2. Interactions among mutations affecting spontaneous mutation, mitotic recombination, and DNA repair in yeast. Montelone BA, et al. Curr Genet, 1995 Jan. PMID 7788712
    3. Allelism of Saccharomyces cerevisiae gene PSO10, involved in error-prone repair of psoralen-induced DNA damage, with SUMO ligase-encoding MMS21. Hoch NC, et al. Curr Genet, 2008 Jun. PMID 18437386
    4. A natural variant of the essential host gene MMS21 restricts the parasitic 2-micron plasmid in Saccharomyces cerevisiae. Hays M, et al. Elife, 2020 Oct 16. PMID 33063663, Free PMC Article
    5. Hyper-recombination and mutator effects of the mms9-1, mms13-1, and mms21-1 mutations in Saccharomyces cerevisiae. Montelone BA, et al. Curr Genet, 1981 Dec. PMID 24185997

    See all (103) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural basis for the E3 ligase activity enhancement of yeast Nse2 by SUMO-interacting motifs.
      Title: Structural basis for the E3 ligase activity enhancement of yeast Nse2 by SUMO-interacting motifs.
    2. Relocation of Collapsed Forks to the Nuclear Pore Complex Depends on Sumoylation of DNA Repair Proteins and Permits Rad51 Association.
      Title: Relocation of Collapsed Forks to the Nuclear Pore Complex Depends on Sumoylation of DNA Repair Proteins and Permits Rad51 Association.
    3. A natural variant of the essential host gene MMS21 restricts the parasitic 2-micron plasmid in Saccharomyces cerevisiae.
      Title: A natural variant of the essential host gene MMS21 restricts the parasitic 2-micron plasmid in Saccharomyces cerevisiae.
    4. Pol2 is the main target of SUMOylation within the replisome and its modification requires the SUMO-ligase Mms21, a subunit of the Smc5/6 complex
      Title: The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε.
    5. These results reveal a mechanism to enhance a SUMO E3 ligase activity by direct DNA binding and to restrict sumoylation in the vicinity of those Smc5/6-Nse2 molecules engaged on DNA.
      Title: DNA activates the Nse2/Mms21 SUMO E3 ligase in the Smc5/6 complex.
    6. Mms21 prevents the accumulation of spontaneous DNA lesions that cause diverse gross chromosomal rearrangements.
      Title: SUMO E3 ligase Mms21 prevents spontaneous DNA damage induced genome rearrangements.
    7. Our findings reveal a requirement for the Smc5/6 complex, including Mms21/Nse2 mediated sumoylation, and topoisomerase-1 (Top1), for maintaining stability of a chromosome having low origin density and suggest a functional cooperation between the Smc5/6 complex and Top1 in maintenance of topologically challenged chromosomes prone to replication fork collapse or accumulation of torsional stress
      Title: Genetic evidence for functional interaction of Smc5/6 complex and Top1 with spatial frequency of replication origins required for maintenance of chromosome stability.
    8. Our findings reveal a functional interplay between Mms21 and Top1 in maintenance of longer chromosomes, and suggest that lack of sumoylation of Mms21 targets coupled with Top1 deficiency is a crucial requirement for accurate inheritance of longer chromosomes.
      Title: Interplay between Top1 and Mms21/Nse2 mediated sumoylation in stable maintenance of long chromosomes.
    9. mms21RINGDelta mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; We identified RPL19A as a novel genetic suppressor of the mms21RINGDelta mutant; Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress
      Title: Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae.
    10. double-strand break relocation to the nuclear envelope depends on SUMOylation mediated by the E3 ligases Siz2 and Mms21
      Title: PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL.
    Submit: New GeneRIF Correction See all GeneRIFs (19)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables SUMO ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables SUMO transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables SUMO transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables SUMO transferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase inhibitor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA double-strand break attachment to nuclear envelope IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair via homologous recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in double-strand break repair via homologous recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein sumoylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein sumoylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of Smc5-Smc6 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of Smc5-Smc6 complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Smc5-Smc6 complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    SUMO ligase MMS21
    NP_010896.3
    • Highly conserved SUMO E3 ligase subunit of SMC5-SMC6 complex; required for anchoring dsDNA breaks to the nuclear periphery; SMC5-SMC6 plays a key role in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; required for efficient sister chromatid cohesion; mutants are sensitive to MMS, show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function; supports nucleolar function

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001137.3 Reference assembly

      Range
      120498..121301 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178834.3 → NP_010896.3  TPA: SUMO ligase MMS21 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010896.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D3DLN0, P38632
      UniProtKB/TrEMBL
      A6ZQT0, B3LRZ7, B5VHB7, C7GX24, C8Z6X9, G2WCL3, N1P3U5
      Conserved Domains (1) summary
      COG5627
      Location:1 → 267
      COG5627; SUMO ligase MMS21, Smc5/6 complex, required for cell growth and DNA repair [Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38632.2

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