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    ROK1 RNA-dependent ATPase ROK1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852704, updated on 2-Nov-2024

    Summary

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    Official Symbol
    ROK1
    Official Full Name
    RNA-dependent ATPase ROK1
    Primary source
    SGD:S000003139
    Locus tag
    YGL171W
    See related
    AllianceGenome:SGD:S000003139; FungiDB:YGL171W; VEuPathDB:YGL171W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables RNA helicase activity. Involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and snoRNA localization. Located in nucleolus. Part of 90S preribosome. Orthologous to human DDX52 (DExD-box helicase 52). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See ROK1 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (182390..184084)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene chromatin-binding exonuclease XRN1 Neighboring gene FG-nucleoporin NUP49 Neighboring gene Spo74p Neighboring gene tRNA-Lys

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Upstream open reading frames regulate the cell cycle-dependent expression of the RNA helicase Rok1 in Saccharomyces cerevisiae. Jeon S, et al. FEBS Lett, 2010 Nov 19. PMID 20969870
    2. Characterization and intracellular localization of the Rok1 protein involved in yeast cell division. Rhee JY, et al. Mol Cells, 1998 Feb 28. PMID 9571634
    3. ATP hydrolysis activity of the DEAD box protein Rok1p is required for in vivo ROK1 function. Oh JY, et al. Nucleic Acids Res, 1999 Jul 1. PMID 10373593, Free PMC Article
    4. A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase. Martin R, et al. RNA, 2014 Aug. PMID 24947498, Free PMC Article
    5. Cofactor-dependent specificity of a DEAD-box protein. Young CL, et al. Proc Natl Acad Sci U S A, 2013 Jul 16. PMID 23630256, Free PMC Article

    See all (72) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that ATP-bound DEAD-box protein Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 protein binding to 40S ribosomes.
      Title: The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.
    2. findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1; Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation
      Title: Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae.
    3. Rrp5 provides specificity to the otherwise nonspecific biochemical activities of the Rok1 DEAD-domain.
      Title: Cofactor-dependent specificity of a DEAD-box protein.
    4. Rok1 was required specifically for the release of snR30.
      Title: Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase.
    5. Immunoprecipitation assays demonstrate the association of Rok1p with 35S pre-rRNA in pre-ribosomes.
      Title: U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35S pre-rRNA, but Rrp5p is essential for association of Rok1p.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on RNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maturation of SSU-rRNA IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in maturation of SSU-rRNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in maturation of SSU-rRNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in snoRNA localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of 90S preribosome HDA PubMed 
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    RNA-dependent ATPase ROK1
    NP_011344.1
    • RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      182390..184084
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181036.1 → NP_011344.1  TPA: RNA-dependent ATPase ROK1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011344.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A6ZU15, D6VTY1, P45818
      UniProtKB/TrEMBL
      B3LHM3, C7GPN6, C8Z880, N1P4C1
      Conserved Domains (2) summary
      COG0513
      Location:101 → 560
      SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
      cd17957
      Location:133 → 336
      DEADc_DDX52; DEAD-box helicase domain of DEAD box protein 52
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P45818.1

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