UBC1 E2 ubiquitin-conjugating protein UBC1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: UBC1
Official Full Name: E2 ubiquitin-conjugating protein UBC1
Primary source: SGD:S000002584
Locus tag: YDR177W
See related: AllianceGenome:SGD:S000002584; FungiDB:YDR177W; VEuPathDB:YDR177W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables proteasome binding activity and ubiquitin-protein transferase activity. Involved in ERAD pathway; ubiquitin-dependent protein catabolic process; and vesicle organization. Located in cytosol. Orthologous to human UBE2K (ubiquitin conjugating enzyme E2 K). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See UBC1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (816878..817525)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubc1 turnover contributes to the spindle assembly checkpoint in Saccharomyces cerevisiae.
Title: Ubc1 turnover contributes to the spindle assembly checkpoint in Saccharomyces cerevisiae.
Cdc34 and Ubc1 are both sufficient for promoting San1 activity, San1 functions preferentially with Ubc1, including when both Ubc1 and Cdc34 are present.
Title: The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control.
the UBA domain of Ubc1 ensures efficient polyubiquitination of substrate by balancing Ubc1 affinity with that of Ubc4
Title: An E2 accessory domain increases affinity for the anaphase-promoting complex and ensures E2 competition.
Tolerance to thermal and reductive stress is regulated by MAP kinase-mediated phosphorylation of Ubc1 at S97 and S115.
Title: Tolerance to thermal and reductive stress in Saccharomyces cerevisiae is amenable to regulation by phosphorylation-dephosphorylation of ubiquitin conjugating enzyme 1 (Ubc1) S97 and S115.
Results suggest that the active site of Ubc1, as well as the surface of ubiquitin, contains specificity determinants that channel specific lysines to the central residues involved directly in catalysis.
Title: Catalysis of lysine 48-specific ubiquitin chain assembly by residues in E2 and ubiquitin.
structure of the flexible class II ubiquitin-conjugating enzyme Ubc1
Title: Solution structure of the flexible class II ubiquitin-conjugating enzyme Ubc1 provides insights for polyubiquitin chain assembly.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables proteasome binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: E2 ubiquitin-conjugating protein UBC1

NP_010462.3

EC 2.3.2.23

Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

816878..817525

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180484.3 → NP_010462.3 TPA: E2 ubiquitin-conjugating protein UBC1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010462.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSF9, P21734

UniProtKB/TrEMBL

A6ZYD5, B3LGA6, B5VG72, C7GN20, C8Z590, G2WAS5, N1P6H1

Conserved Domains (2) summary

pfam09288
Location:161 → 215

UBA_3; Fungal ubiquitin-associated domain

COG5078
Location:1 → 151

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]