UBC9 E2 SUMO-conjugating protein UBC9 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: UBC9
Gene description: E2 SUMO-conjugating protein UBC9
Primary source: FungiDB:YDL064W
Locus tag: YDL064W
See related: SGD:S000002222; AllianceGenome:SGD:S000002222; VEuPathDB:YDL064W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables SUMO transferase activity. Involved in mitotic spindle elongation and protein sumoylation. Located in condensed nuclear chromosome. Used to study cancer. Orthologous to human UBE2I (ubiquitin conjugating enzyme E2 I). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See UBC9 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (337487..338070)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Replacement of proline with leucine at position 123 induces a switch from the preferential addition of SUMO to lysine residues in unstructured sites to acceptor lysines embedded in secondary structures. This causes alterations in SUMO chain linkages.
Title: UBC9 Mutant Reveals the Impact of Protein Dynamics on Substrate Selectivity and SUMO Chain Linkages.
Ubc9 sumoylation is a key event for meiotic synaptonemal complex formation. SUMO Ubc9 enhances catalysis by binding and orienting donor SUMO charged Ubc9.
Title: Ubc9 sumoylation controls SUMO chain formation and meiotic synapsis in Saccharomyces cerevisiae.
The crystal structure of sumoylated Ubc9 demonstrates how the newly created binding interface can provide a gain in affinity otherwise provided by E3 ligases.
Title: Ubc9 sumoylation regulates SUMO target discrimination.
These findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress.
Title: Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress.
analysis of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p
Title: Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a non-covalent ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables SUMO conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	IEA Inferred from Electronic Annotation more info
involved_in protein sumoylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E2 SUMO-conjugating protein UBC9

NP_010219.1

SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

337487..338070

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180123.1 → NP_010219.1 TPA: E2 SUMO-conjugating protein UBC9 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010219.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRT3, P50623

UniProtKB/TrEMBL

A6ZXR2, B3LGW1, C8Z4K5, G2WC97, N1P473

Conserved Domains (1) summary

COG5078
Location:1 → 157

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]