ATG18 phosphoinositide binding protein ATG18 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ATG18
Official Full Name: phosphoinositide binding protein ATG18
Primary source: SGD:S000001917
Locus tag: YFR021W
See related: AllianceGenome:SGD:S000001917; FungiDB:YFR021W; VEuPathDB:YFR021W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AUT10; CVT18; NMR1; SVP1
Summary: Enables phosphatidylinositol phosphate binding activity and ubiquitin binding activity. Involved in several processes, including macroautophagy; vacuolar protein processing; and vacuolar transport. Located in several cellular components, including fungal-type vacuole membrane; phagophore; and phagophore assembly site. Part of PAS complex. Orthologous to human WIPI1 (WD repeat domain, phosphoinositide interacting 1) and WIPI2 (WD repeat domain, phosphoinositide interacting 2). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See ATG18 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (194812..196314)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vacuole fragmentation depends on a novel Atg18-containing retromer-complex.
Title: Vacuole fragmentation depends on a novel Atg18-containing retromer-complex.
The crystal structure of Atg18 reveals a new binding site for Atg2 in Saccharomyces cerevisiae.
Title: The crystal structure of Atg18 reveals a new binding site for Atg2 in Saccharomyces cerevisiae.
Atg2-Atg18 complex tethers the preautophagosomal structure to the endoplasmic reticulum to initiate membrane expansion during autophagosome formation.
Title: The Atg2-Atg18 complex tethers pre-autophagosomal membranes to the endoplasmic reticulum for autophagosome formation.
ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Title: ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Data provide a model whereby the Atg18 beta-propeller provides organelle specificity by binding to two determinants on the target membrane.
Title: Atg18 function in autophagy is regulated by specific sites within its β-propeller.
The Atg2-Atg18 complex and Atg9 localized to the edge of the IM, appearing as two or three dots, in close proximity to the endoplasmic reticulum exit sites.
Title: Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.
effective targeting of Atg2 to the PAS can compensate for loss of Atg18 function in autophagy
Title: Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2.
Deletion of ATG18, ATG21 and YGR223c, alone or simultaneously has no obvious function on the MVB-pathway and carboxypeptidase Y sorting.
Title: Dissecting the localization and function of Atg18, Atg21 and Ygr223c.
Atg18 forms a complex with Atg2 irrespective of PtdIns(3)P binding, associates tightly to autophagic membranes by interacting with PtdIns(3)P, and plays an essential role.
Title: The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function.
Atg18p is likely a PtdIns(3,5)P(2)"sensor," acting as an effector to remodel membranes as well as regulating its synthesis via feedback that might involve Vac7p
Title: Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-3,5-bisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy of mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy of peroxisome	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pexophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vacuole organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein lipidation involved in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to phagophore assembly site	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vacuolar protein processing	IDA Inferred from Direct Assay more info	PubMed
involved_in vacuolar protein processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of PAS complex	IDA Inferred from Direct Assay more info	PubMed
part_of PAS complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cell periphery	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in phagophore	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
is_active_in phagophore assembly site membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in phagophore assembly site membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: phosphoinositide binding protein ATG18

NP_444297.1

Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (CVT) pathway; binds PtdIns(3,5)P2, PI3P and PI4P; WD-40 repeat-containing protein and PROPPIN family member; relocalizes from the vacuole to cytoplasm upon replication stress; forms higher order helical assemblies; mammalian homologs include: WIPI1, WIPI2, WIPI3 and WDR45; human WDR45 mutations cause static encephalopathy of childhood with neurodegeneration in adulthood (SENDA)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

194812..196314

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179986.1 → NP_444297.1 TPA: phosphoinositide binding protein ATG18 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_444297.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A7A258, D6VTQ1, P43601

UniProtKB/TrEMBL

B3LUN0, B5VI48, C7GYC6, C8Z7V6, G2WDF9, N1PA29

Conserved Domains (2) summary

sd00039
Location:249 → 285

7WD40; WD40 repeat [structural motif]

cl29593
Location:224 → 318

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...