U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    BUD32 serine/threonine protein kinase BUD32 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853178, updated on 2-Nov-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    BUD32
    Official Full Name
    serine/threonine protein kinase BUD32
    Primary source
    SGD:S000003494
    Locus tag
    YGR262C
    See related
    AllianceGenome:SGD:S000003494; FungiDB:YGR262C; VEuPathDB:YGR262C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    LDB14
    Summary
    Enables ATP hydrolysis activity and protein serine/threonine kinase activity. Involved in positive regulation of transcription by RNA polymerase II; tRNA threonylcarbamoyladenosine metabolic process; and telomere maintenance via recombination. Located in nucleus. Part of EKC/KEOPS complex. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome 4. Orthologous to human TP53RK (TP53 regulating kinase). [provided by Alliance of Genome Resources, Nov 2024]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See BUD32 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (1016974..1017759, complement)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Tna1p Neighboring gene Apl6p Neighboring gene steryl deacetylase Neighboring gene methionine--tRNA ligase MES1

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. Stocchetto S, et al. FEBS Lett, 1997 Sep 1. PMID 9305753
    2. Structure-function analysis of yeast piD261/Bud32, an atypical protein kinase essential for normal cell life. Facchin S, et al. Biochem J, 2002 Jun 1. PMID 12023889, Free PMC Article
    3. Acidophilic character of yeast PID261/BUD32, a putative ancestor of eukaryotic protein kinases. Facchin S, et al. Biochem Biophys Res Commun, 2002 Sep 6. PMID 12207926
    4. Functional homology between yeast piD261/Bud32 and human PRPK: both phosphorylate p53 and PRPK partially complements piD261/Bud32 deficiency. Facchin S, et al. FEBS Lett, 2003 Aug 14. PMID 12914926
    5. Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity. Li QX, et al. Biochem J, 2022 Dec 9. PMID 36416748

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. the crystal structure of Bud32/Cgi121 in complex with ADP revealed that ADP is bound in the catalytic site of Bud32 in a canonical manner
      Title: Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.
    2. EKC/KEOPS complex is specifically involved in the regulation of Bud9p localization downstream of Rax1p/Rax2p.
      Title: Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
    3. Sch9 phosphorylates, both in vitro and in vivo, Ser258 of Bud32. This modification does not affect the catalytic properties of Bud32, but promotes its ability to associate with its substrate Grx4p and, consequently, to phosphorylate it.
      Title: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ATP hydrolysis activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables histone H2AS1 kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular bud site selection HMP PubMed 
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of transcription by RNA polymerase II IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in protein phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of EKC/KEOPS complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of EKC/KEOPS complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, telomeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    serine/threonine protein kinase BUD32
    NP_011778.1
    • Component of KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; provides the ATPase and GTPase activities of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human ortholog TP53RK

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      1016974..1017759 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181391.1 → NP_011778.1  TPA: serine/threonine protein kinase BUD32 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011778.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VV41, P53323
      UniProtKB/TrEMBL
      A6ZUS0, B3LHY0, B5VJK4, C7GNH0, C8Z9F4, G2WEY7, N1P4G6
      Conserved Domains (1) summary
      COG3642
      Location:19 → 261
      Bud32; tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53323.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools